Expression of Ezrin in Vagina Cells of Postmenopausal Rats after Dietary Administration of Omega-3 Fatty Acid Formula

다국어 초록 (Multilingual Abstract)

To see the effect of dietary administration of omega 3-fatty acid formula on the vaginal cells of postmenopausal rats.
Methods: Three-week-old female Wistar/ST rats were raised after one week of adjustment period. The rats were then dividedinto three groups, for three different kinds of diet; general diet, 1% omega-3 fatty acid diet, and 2% omega-3 fatty acid diet. Aftereight weeks of having assigned diet, after the oophorectomy, with the same diet previously they had Immunohistochemistry,Immunofluorescence, and Western Blot about ezrin, merlin were done.
Results: In immunohistochemistry, estrogen injection group revealed thicker and well differentiated features. In Immunofluorescence,Omega-3 fatty acid composition in diet did not effect expression of ezrin and merlin in rat vagina in estrogeninjection group, their vaginal epithelium showed full layers (from basal to apical layer). In Western Blot analysis, Omega-3 fatty acidcomposition in diet did not affect expression of ezrin and merlin in rat vagina estrogen presented significant impact on expressionof ezrin and merlin.
Conclusion: Although omega-3 fatty acid composition changed in diet, vaginal epithelial morphology unchanged. Estrogen dideffect on vagina cell, but omega-3 fatty acid did not effect on ezrin and merlin in vagina. (J Menopausal Med 2014;20:97-103)

참고문헌 (Reference)

1 김준모, "폐경 후 요로계 변화와 폐경호르몬치료 효과" 대한폐경학회 17 (17): 136-141, 2011

2 김태희, "폐경 쥐 모델에서 두류 섭취에 따른 자궁의 비타민 D 수용체의 발현" 대한폐경학회 19 (19): 1-8, 2013

3 허주미, "폐경 여성의 영양소 및 식품 섭취 상태 평가" 대한폐경학회 17 (17): 12-20, 2011

4 김태희, "폐경 여성에서의 오메가-3 지방산" 대한폐경학회 18 (18): 75-80, 2012

5 김대경, "유통중인 건강기능식품의 감마-리놀렌산 및 오메가-3 지방산 함량 모니터링" 농업과학연구소 38 (38): 277-284, 2011

6 Skala CE, "The effect of hormonal status on the expression of estrogen and progesterone receptor in vaginal wall and periurethral tissue in urogynecological patients" 153 : 99-103, 2010

7 Moon HJ, "Positive correlation between erythrocyte levels of n-3 polyunsaturated fatty acids and bone mass in postmenopausal Korean women with osteoporosis" 60 : 146-153, 2012

8 Curto M, "Ezrin...a metastatic detERMinant?" 5 : 113-114, 2004

9 Hiscox S, "Ezrin regulates cell-cell and cellmatrix adhesion, a possible role with E-cadherin/betacatenin" 112 : 3081-3090, 1999

10 Fadiel A, "Ezrin is a key element in the human vagina" 60 : 31-41, 2008

11 Song J, "Estradiol-induced ezrin overexpression in ovarian cancer: a new signaling domain for estrogen" 220 : 57-65, 2005

12 Arpin M, "Emerging role for ERM proteins in cell adhesion and migration" 5 : 199-206, 2011

13 Kim TH, "Adhesions after gynecologic surgery in postmenopausal women" 16 : 134-141, 2010

14 Zheng S, "17beta-Estradiol enhances breast cancer cell motility and invasion via extra-nuclear activation of actin-binding protein ezrin" 6 : 2011