홍화가 위장관 카할간질세포에 미치는 효과

다국어 초록 (Multilingual Abstract)

The purpose of this study is to investigate the effects of Carthami Flos on interstitial cells of Cajal in the gastrointestinal tract. Many regions of the tunica muscularis of the gastrointestinal (GI) tract display spontaneous contraction. These spontaneous contractions are mediated by periodic generation of electrical slow waves. Recent studies have shown that the interstitial cells of Cajal (ICCs) act as pacemakers and conductors of electrical slow waves in gastrointestinal smooth muscles. We investigated the cytotoxicity activity, antioxidant activity, and pacemaking activity. The cytotoxicity activity was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Antioxidant activities were determined by DPPH (1.1-diphenyl-2-picrylhydrazyl) radical scavenging capacity assay and DCFH-DA (2,7-dichlorofluorescein diacetate) method. The effects of Carthami Flos on the pacemaker potentials in cultured ICCs from murine small intestine were investigated by using whole-cell patch-clamp techniques at 30℃. The addition of Carthami Flos (5, 10, 30 μg/ml) depolarized the resting membrane potentials in a concentration dependent manner. These results suggest that the GI tract can be targets for Carthami Flos, and their interaction can affect intestinal motility.

참고문헌 (Reference)

1 이광현, "홍화씨로부터 추출된 세포토닌계 화합물 및 이를 함유하는 조성물"

2 이인우, "홍화씨 건강법" 태일출판사 45-70, 1998

3 최성희, "홍화꽃 및 홍화씨의 휘발성성분" 한국식품과학회 14 (14): 36-42, 2004

4 최영주, "홍화(Carthamus tinctorius L.)씨의 sterol 및 phytoestrogen 분석" 한국생명과학회 13 (13): 529-534, 2003

5 한국생약교수협의회, "본초학" 사단법인 약사회 553-555, 1995

6 최한기, "고농서국역총서11-농정회요Ⅱ (農政會要)" 농촌진흥청 271-277,

7 Huizinga, J.D., "W/kit gene required for interstitial cells of Cajal and for intestinal pacemaker activity" 373 : 347-349, 1995

8 Iwata, T., "The effect of dietary safflower phospholipid on sterols in gastrointestinal tract of rats fed a hypercholesterolemic diet" 38 : 615-622, 1992

9 Kee, C.H, "The Pharmacology of Chinese Herbs" CRC press 249-250, 1993

10 Cajal, S.R, "Sur les ganglions et plexus nerveux de I'intestin" 45 : 217-223, 1893

11 Koh, S.D., "Spontaneous electrical rhythmicity in cultured interstitial cells of cajal from the murine small intestine" 513 : 203-213, 1998

12 Takii, T., "Serotonin derivative, N-(p-Coumaroyl)serotonin, isolated from safflower (Carthamus tinctorius L.) oil cake augments the proliferation of normal human and mouse fibroblasts in synergy with basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF)" 125 : 910-915, 1999

13 Tokutomi, N., "Rhythmic Cl- current and physiological roles of the intestinal c-kit positive cells" 431 : 169-177, 1995

14 Kim, M.N., "Researches for analgesic andhepatoprotective action of Carthami Flos" 26 : 32-36, 1992

15 Zhang, H.L., "Novel antioxidants from safflower (Carthamus tinctorius L.)" 44 : 874-876, 1996

16 Farrugia, G, "Ionic conductances in gastrointestinal smooth muscles and interstitial cells of Cajal" 61 : 45-84, 1999

17 Ward, S.M., "Interstitial cells of Cajal mediate enteric inhibitory neurotransmission in the lower esophageal and pyloric sphincters" 115 : 314-329, 1998

18 Cleary, M.P., "Genotype and diet effects in lean and obese Zucker rats fed either safflower or coconut oil diets" 220 : 152-161, 1999

19 Szurszewski, J.H, "Electrical basis for gastrointestinal motility. In Physiology of the gastrointestinal tract, 2nd edn, ed" Raven Press 383-422, 1987

20 Tomita, T, "Electrical activity (spikes and slow waves) in gastrointestinal smooth muscles. In Smooth Muscle: An Assessment of Current Knowledge" Edward Arnold 127-156, 1981

21 Cox, C., "Effects of coconut oil, butter, and safflower oil on lipids and lipoproteins in persons with moderately elevated cholesterol levels" 36 : 1787-1795, 1995

22 Heo J, "Dong-I Bogam 5. 3th eds, In Safflower" Yekang Publishers 2763-2764, 1986

23 김희정, "Bone-protecting effect of safflower seeds in ovariectomized rats" SPRINGER 71 (71): 88-94, 2002

24 Zhang, H.L., "Antioxidative compounds isolated from safflower (Carthamus tinctorius L.)" 45 (45): 1910-1914, 1997

25 Kim, M.J., "Anti-wrinkle effect of safflower seed extract (I)" 30 : 15-20, 2004

26 김미진, "Anti-wrinkle Effect of Safflower (Carthamus tinctorius) Seed Extract (II)" 30 (30): 449-456, 2004

27 김미진, "Anti-wrinkle Effect of Safflower (Carthamus tinctorius) Seed Extract (I)" 30 (30): 15-22, 2004

28 Sanders, K.M, "A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract" 111 : 492-515, 1996