피부결핵의 조직에서 중합효소 연쇄반응을 이용한 Mycobacterium tuberculosis DNA의 검색에 관한 연구 = Detection of Mycobacterium tuberculosis DNA using the Polymerase Chain Reaction in Paraffin - embedded Biopsy Specimens of Skin tuberculosis피부결핵의 조직에서 중합효소 연쇄반응을 이용한 Mycobacterium tuberculosis DNA의 검색에 관한 연구

다국어 초록 (Multilingual Abstract)

Beckground : A presumptive diagnosis of cutaneous tuberuisis can be made on the basis of patients history, clinical and histologic findings. However, isolation of Mycabecterum tuberculosis is required for a defimitive diagnosis and confirmation carke difficult with traditional techniques, including the direct visoalization in tissue and growth culture. Objective : We used polymerase chain reaction(PCR) for the detection of mycobacterial DNA from routinely prepared formalin-fixed, paraffin-embedded skin specimens to evaluate its usefulness as a diagnrstic tool Method : We studied typical cases of localized cutaneous rerculosis. Ten paraffin-embedded biopsy samples obtained from 2 patients with scrofulo lupus vulgaris and tuberculosis verrucosa cutis were analyzed by PCR, respectively. Result : M. tubereuloeis DNA was demonstrated in all 10 specimens. Cenclusion : This study shows that the PCR will be a useful method for the confirmation of the diagnosis in localizeted cutaneous tuberculosis and many sl in lesions thought to be related fo tuberculosia infection.
(Kor J Dermatol 1994; 32(6): 990-997)