Exploring Effects of Calorie Restriction on Aging in Subcutaneous Adipose Tissue = 칼로리 제한이 피하지방 조직에서 노화에 미치는 영향 연구

목차 (Table of Contents)

• ABSTRACT i
• 국문 초록 iii
• TABLE OF CONTENTS v
• LIST OF TABLES vii
• LIST OF FIGURES viii
• NOMENCLATURE ix
• CHAPTER 1. INTRODUCTION 1
• CHAPTER 2. MATERIALS AND METHODS 3
• 2.1 Data collection and processing 3
• 2.1.1 GTEx bulk RNA-seq data 3
• 2.1.2 CR bulk RNA-seq data 3
• 2.1.3 Mouse brown adipose tissue bulk RNA-seq data 6
• 2.2 Identification of aging gene set for 31 tissues 6
• 2.3 Deconvolution of bulk RNA-seq data 7
• 2.4 Enrichment analysis 7
• 2.5 Identification of gene level and pathway level association 8
• 2.6 Construction of co-expression network 9
• 2.7 Upstream regulator analysis 9
• CHAPTER 3. RESULTS 10
• 3.1 Aging effect in SAT 10
• 3.2 Transcriptomic landscape of aging in SAT 12
• 3.2.1 Proportion of cell types for aging in SAT 12
• 3.2.2 Identification of function of aging-associated genes from SAT 12
• 3.2.3 Identification of enriched pathways in aging SAT 13
• 3.3 Transcriptomic landscape of CR in SAT 19
• 3.3.1 Proportion of cell types for CR in SAT 19
• 3.3.2 Identification of function of DEGs from CR in SAT 19
• 3.3.3 Identification of enriched pathways in CR 20
• 3.4 Gene level association between aging and CR, and specific function of the overlapping genes 26
• 3.4.1 Identification of gene level association 26
• 3.4.2 Specific function of UAG-CRD and DAG-CRU 26
• 3.5 Pathway level association between aging and CR, and detection of enriched metabolic pathway 29
• 3.5.1 Identification of pathway level association 29
• 3.5.2 Detection of enriched metabolic pathway 29
• 3.6 Identification of hub genes and upstream regulators of CR intervention for aging 32
• 3.6.1 Identification of hub genes 32
• 3.6.2 Identification of upstream regulators 32
• 3.6.3 Identification of sex-specific manner of KLF15 33
• 3.7 Identification of function of KLF15 in adipose tissue 41
• 3.8 Conformation of association between aging and CR with cellular senescence 42
• CHAPTER 4. DISCUSSION 43
• REFERENCES 46