Adenosine Deaminase를 생성하는 방선균의 특성 = Characteristics of and Adenosin Deaminase-Producing Actinomycete

국문 초록 (Abstract)

Purine nucleotide의 대사계에 관련하는 aminohydrolase가운데adenosine을 탈아미노화 시켜 inosine으로의 전환을 촉매하는 adenosine deaminase는 동물기원이 대부분이다. 따라서 미생물기원의 효소에 대한 연구보고는 희소하며 특별히 세포내 효소가 아닌 세포의 효소로서의 adenosine deaminase산생 미생물에 대한 보고는 극히 미진하다. 그러므로 본 실험연구는 세포의 adenosine deaminase를 산생하는 방선균을 검색하고 그 분류학적 특성을 검토하는데 그 목표를 두었다.

다국어 초록 (Multilingual Abstract)

An actinomycete able to produce an extracellular adenosine deaminase catalyzing the conversion of adenosine to inosine by the deamination was isolated from sold samples. Examinations were made through the microscopic observation of shape, color of aerial and substrate mycelium, presence of soluble pigments, and assimilation of sugars. As the results obtained, the isolate showed good growth on the known test-media used for the classification of actinomycetes, and did simply branched-aerial hyphae with white color In general. The spore-bearing hyphae of isolate possessed spore chain and the color of substrate was brown, or yellow. Soluble pigments were also produced.
The isolate produced the melanin pigment on peptone-yeast extractiron agar and presented high level of activity in gelatin liquefaction and nitrate reduction. From the above charactenstics and the presence of L,L-diaminopimelic acid as component of cell wall, she was identified genus Streptomyces and named Streptomyces sp. Y-225.

목차 (Table of Contents)

• Ⅰ.머리말
• Ⅱ.재료 및 방법
• Ⅲ.결과 및 고찰
• Ⅳ.맺음말