유리 산소 기 청소제가 자외선 B에 의한 생쥐 표피 Langerhans 세포의 ATPase 표현에 미치는 영향 = The Effect of Free Oxygen Radical Scavengers on the Expression of ATPase of Mouse Epidermal Langerhans Cells After UVB Irradiation유리 산소 기 청소제가 자외선 B에 의한 생쥐 표피 Langerhans 세포의 ATPase 표현에 미치는 영향

다국어 초록 (Multilingual Abstract)

The Effect of Free Oxygen Radical Scavengers on the Expression of ATPase of Mouse Epidermal Langerhans Cells After UVB Irradiation Seong Jin Jeon, M.D., Keo Suck Suh, M.D., Sang Tae Kim, M.D. Department of Dermatology, Kosin Medical College, Pusan, Korea Langerhans cells are dendritic cells with specific granules in their cytoplasm, and Ia antigen and receptors for Fc portion of IgG and C3 on their cell surface. They are known to perform immunological functions such as processing and presentation of antigen to T cells in delayed type contact hypersensitivity, and surveillance of virus and cancer. It is known that Langerhans cells are damaged functionally and morphologically by UVB irradiation, but the mechanisms are still not known or are largely speculative, though keratinocytes are known to be damaged by reactive oxygen species generated by UV irradiation. It was the investigator`s idea that reactive oxygens generated by UVB exposure on skin could also play a role in Langerhans cell damage and that free oxygen radical scavengers would prevent the damage of Langerhans cells from UVB. Free radical scavengers used in this experiments were superoxide dismutase(SOD), α-tocopherol acetate(α-TCA) and sodium azide(SA), and these chemicals were known to inactivate O_2^-, OH and 1^O_2 respectively. Groups of BALB/c mice were injected intraperitoneally wth 0.2㎖ of SOD(0.1 or 1.0mg/㎖), α-TCA(10^4 or 10^2mole) or SA(10^4 or 10^2mole) just before or immediately after UVB irradiation(40mJ/㎠). Control mice given saline, saline plus UVB irradiation or free oxygen radical scavengers without UVB. Seventy two hours after the irradiation of UVB, biopsy specimens were taken from the ears of mice. Number, area and perimeter of Langerhans cells were evaluated using ATPase-stained epidermal sheets. The results were as follows : 1. The number of Langerhans cells was significantly decreased by UVB irradiation(p<0.01). 2. The area and perimeter of Langerhans cells were significantly increased by UVB irradiation(p<0.01). 3. SOD could prevent Langerhans cell damage significantly from UVB(p<0.05). 4. For α-TCA, only high dose(10^2mole) and treatment immediately before UVB irradiation showed protective effect from Langerhans cell damage(p<0.05). 5. SA had no protective effect regardless of the doses or time of administration(p>0.05).