유기용매계에서의 리파제 촉매반응에 의한 에스테르 교환 반응 = Lipase-CAtalyzed Ester Exchange Reaction in Organic Solvent

국문 초록 (Abstract)

유기용매계에서 lipase를 이용한 ester교환반응 시 효소활성과 반응 안정성에 미치는 인자등에 대해 조사하여 반응 조건의 최적화를 시도하였다. palmitic acid methylester와 lauryl alcohol를 기질로 사용하여 에스테르 교환 반응에의해 lauryl palmitate를 합성하는 반응조건은 Candida cylindracea lipase의 경우 cyclohexane에 0.6% 물을 첨가하고 최적 온도인 35˚C에서 80시간 반응 시켰으며 그 결과 92%의 ester교환방응이 일어났다. Porcine pancreatic lipase의 경우에는 cyclohexane에 0.6% 물을 첨가하고 최적온도인 45˚C에서 80시 간 반응시킨 결과 82%라고 하는 고수율의 lauryl palmitate가 합성되었다.

다국어 초록 (Multilingual Abstract)

Ester exchange reaction between palmitic acid methyl ester and lauryl alcohol was investigated in organic solvent using lipase. As a result, hexane, toluene, cyclohexane, and isooctane were adequate for lipase-catalyzed ester exchange reaction. These solvents were used to investigate optimum activity condition and examine the time course of ester exchange reation in optimal conditions. Enzyme activity in organic solvent increased in proportion with water content up to 0.6%(V/V). The optimum temperature for the reaction was 35˚C and 45˚C for the Candida cylindracea lipase and porcine pancreatic lipase, respectively. Thermal stability of lipase in organic solvent was found to be higher than that in water. At the optimized conditions, conversion yield of lauryl palmitate after 80hrs of reaction was 92% and 82% for the Candida cylindracea lipase and porcine pancreatic lipase, respectively.