백화고 생산을 위한 표고버섯 종균 자원의 보존 및 기능성 특성 연구

다국어 초록 (Multilingual Abstract)

Preservation and Functional Properties of Shiitake Mushroom (Lentinula edodes) Spawn Resources for Baekhwago Production Baek Hyun Advisor : Prof. Beak Jihwan, Ph.D. Department of Food and Nutrition, Graduate School of Gwangju University The purpose of this study was to obtain fundamental data for the development of domestic mushroom mycelia strains suitable for local cultivation environments, thereby reducing reliance on imported mushroom strains. Mushrooms cultivated in a smart farm system were classified into small (S), medium (M), and large (L) groups based on weight. Mycelia strains were aseptically isolated and cultivated in a liquid medium containing potassium, magnesium sulfate, sucrose, and soybean meal at 25℃. After cultivation, nucleotide sequence analysis was performed, and a commercially available Lentinula edodes strain obtained from the Korean Agricultural Culture Collection (KACC) was used as a reference strain. Sequence analysis identified most isolates as Lentinula edodes, after which functional component analyses were conducted. In the DPPH radical scavenging assay, mycelia exhibited IC₅₀ values ranging from 77.23 to 84.19µg/mL, while liquid culture extracts showed IC₅₀ values of 81.86 to 185.09µg/mL, indicating relatively higher antioxidant activity in the mycelia samples. Similar trends were observed in the ABTS radical scavenging assay, with mycelia ranging from 0 to 188.45µg/mL and liquid culture extracts from 0 to 160.74µg/mL. Among all groups, the L group demonstrated the highest antioxidant activity. β-Glucan content ranged from 0.03 to 0.72mg/g in mycelia and from 7.36 to 14.55mg/g in liquid cultures, indicating significantly enhanced accumulation under liquid culture conditions. Total polyphenol content was also higher in liquid cultures (3.52–15.89mg GAE/mL) compared to mycelia (1.36–2.44mg GAE/g). Further comparison between fruiting bodies and liquid cultures of Baekhwago showed that β-glucan content was 0.68–1.54mg/g in fruiting bodies and 94.86– 98.00mg/g in liquid cultures, while total polyphenol content was 5.68–7.71mg GAE/g and 325.92–357.23mg GAE/g, respectively. These results demonstrate that liquid culture conditions provide a favorable environment for the accumulation of functional bioactive compounds. This study provides valuable baseline data for the development of high-functional Baekhwago-based bio materials and supports the potential of domestic mushroom mycelia strains for functional material applications.

목차 (Table of Contents)

• 제1장 서론 01
• 제1절 서론 01
• 1. 연구의 필요성 및 목적 01
• 2. 시장적·산업적 배경 03
• 3. 기존 연구 및 산업 환경의 한계점 06
• 4. 연구의 차별성 09
• 제2절 이론적 배경 11
• 1. 백화고의 개념 11
• 2. 표고버섯의 생리학적 특성 14
• 3. 국내 버섯 산업의 현황 16
• 4. 수입산 종균 의존 문제 및 기술적 한계 18
• 5. 버섯 기능성 성분의 생화학적 특성· 20
• 5.1. DPPH 및 ABTS radical 소거능 20
• 5.2. 베타글루칸 22
• 5.3. 총 폴리페놀· 24
• 6. 버섯 스마트팜 재배 환경 26
• 제3절 연구의 목적 28
• 제2장 재료 및 방법 30
• 제1절 실험 재료 30
• 1. 표고버섯 백화고 30
• 2. 표고버섯 백화고의 재배환경 조사 32
• 3. KACC 균주 33
• 제2절 실험 방법 34
• 1. 원균 분리 및 배양 34
• 2. 유전자 분석 35
• 3. 기능성 성분 분석 37
• 3.1. DPPH radical 소거능 37
• 3.2. ABTS radical 소거능 39
• 3.3. 베타글루칸 함량 41
• 3.4. 총 폴리페놀 함량 42
• 3.5. 백화고 자실체의 베타글루칸 및 총 폴리페놀 함량 44
• 4. NGS 분석· 45
• 5. 통계 분석 46
• 제3장 결과 및 고찰 47
• 1. 표고버섯 백화고의 재배 환경 조사 47
• 2. 원균 배양 및 유전자 분석 50
• 3. 기능성 성분 분석 56
• 3.1. DPPH radical 소거능 56
• 3.2. ABTS radical 소거능 59
• 3.3. 베타글루칸 함량 62
• 3.4. 총 폴리페놀 함량 65
• 3.5. 백화고 자실체의 베타글루칸 및 총 폴리페놀 함량 68
• 4. NGS 분석· 72
• 제4장 종합 고찰 및 적요 84
• 제5장 요약 86
• 참고문헌 88