Cytotoxicity of ZnO and TiO₂ Nanoparticles Generated by Laser Ablation for the Usage of Sunscreen

다국어 초록 (Multilingual Abstract)

This paper aims to address the cellular toxicity of ultra-pure zinc oxide (ZnO) and titanium dioxide (TiO2) nanoparticles (NPs) frequently employed in sunscreens as inorganic physical sun blockers to provide protection against adverse effects of ultraviolet (UV) radiation including UVB (290-320 nm) and UVA (320-400 nm). In consideration that the production and the use of inorganic NPs have aroused many concerns and controversies regarding their safety and toxicity, it is very important to directly investigate a main problem related to the intrinsic/inherent toxicity of these NPs and/or their incompatibility with biological objects(cancer cells and adults zebrafish). In the present study, we took advantage of the laser-assisted method called laser ablation for generation of ZnO and TiO2 NPs. NPs were prepared through a physical process of irradiating solid targets in liquid phase and we took UV-Vis spectrum, TEM, XRD, PL and EDAX results to prove these nanoparticles’ properties. Our results show that TiO2 NPs are essentially non-poisonous and ZnO NPs are more toxic than TiO2 NPs based on the cell viability assays. And the results for zebrafish show ZnO and TiO2 NPs are not acute because of NPs’ aggregation.

목차 (Table of Contents)

• Ⅰ. 서론 1
• Ⅱ. 본론 4
• 1. 실험 장치 및 실험 방법 4
• 가. 레이저 기화법 (Laser Ablation) 4
• 나. 세포 독성 실험 (MTT assay) 7
• 다. Zebrafish를 이용한 독성 실험 10
• 2. 실험 결과 11
• 가. 세포 독성 실험 (MTT assay) 11
• 1) 레이저 기화법에 의하여 합성된 나노 입자의 성질 (Surelite I) 11
• 2) 레이저 기화법에 의하여 합성된 Ag, Au, Ni, Cr 나노 입자의 세포 독성 실험 결과 21
• 3) 레이저 기화법에 의하여 합성된 ZnO와 TiO2 나노 입자의 세포 독성 실험 결과 26
• 4) 시중에 판매되는 ZnO와 TiO2 나노 입자의 세포 독성 실험 결과 30
• 나. Zebrafish를 이용한 독성 실험 39
• 1) 레이저 기화법에 의하여 합성된 나노 입자의 성질 (YG 980) 39
• 2) Zebrafish를 이용한 독성 실험 결과 41
• Ⅲ. 결론 44
• Ⅳ. 참고 문헌 46