Experiments were performed to find out the proper conditions of diluents for the short-tenn storage of sperm of black seabream, Acanthopagrus schlegeli. For milt collection, brood stock was reared in a recirculating seawater system. The following resu...
Experiments were performed to find out the proper conditions of diluents for the short-tenn storage of sperm of black seabream, Acanthopagrus schlegeli. For milt collection, brood stock was reared in a recirculating seawater system. The following results indicated that short-term storage methods with fresh condition could tie employed in black seabream sperm. When the black seabream's serum was used as a diluent for the fresh storage, sperm activity index (SAI), fertilization rate and survival rate derived the best result in $7\~10\;days$ of storage. pH 7 and 8 showed the highest SAI in the same storage condition. In order to keep high SAI and survival rate of the sperm, addition of 800 ppm neomycine into the diluent revealed the best storage results.