한국인에서 Beta-2 아드레날린 수용체 유전자인 ADRB2의 다형현상과 한국인의 아토피 피부염 발병의 연관성 = Polymorphism of ADRB2 genes, beta-2 adrenoceptor related to atopic dermatitis in Koreans

다국어 초록 (Multilingual Abstract)

This study was to test the genetic association between beta-2 adrenoceptor polymorphisms (+46A>G and +79C>G) and atopic dermatitis(AD) in Korean population. We analyzed in 174 cases of AD and 145 non-AD control DNA sample. At 2 sites of Beta-2 adrenoceptor (ADRB2) gene showed variants and had polymorphisms. The allele frequencies of beta-2 adrenoceptor A-codon 16 had 0.66 in normal control group and 0.79 in atopy dermatitis (AD) group. The allele frequencies of Beta-2 adrenoceptor C-codon 27 had 0.92 in normal control and AD groups, respectively. But codon 16 and codon 27 are no significant differences between normal and AD groups (P=0.952). Thus, it seems that polymorphisms in ADRB2 are not associated with AD development in Koreans.

목차 (Table of Contents)

• 1. 서론
• 2. 재료 및 방법
• 2.1 재료
• 2.2 DNA 추출
• 2.3 프라이머 선택
• 2.4 중합효소 연쇄반응(Polymerase Chain Reaction; PCR)
• 2.5 아가로즈 전기영동 (Agarose Gel Electrophoresis)
• 2.6 Restriction Fragment Length Polymorphism (RFLP)
• 2.7 통계처리분석
• 3. 결과
• 4. 고찰
• 5. 결론