Thickness and Pore Size Control of Chitin Nanofibers by Ultra-sonication and Its Biological Effect in vitro

다국어 초록 (Multilingual Abstract)

Electrospinning is one of widely used methods fabrication of tissue engineering scaffold. Chitin, a natural polymer, is used as biomedical materials due to its biocompatibility and biodegradability. This study is to obtain the chitin nanofibrous scaffold for tissue engineering application. In this study, pore size and thickness of chitin nanofibous sheet controlled by ultra-sonication. The surface properties of chitin nanofibrous sheet and ultra-sonicated scaffold performed scanning electron microscopy and water contact angle. The cellular adhesion and migration on the chitin nanofibrous scaffolds were evaluated by MTT and hematoxylin & eosin staining, respectively. As the chitin nanofibrous sheet had above about 2.4 μm surface pore size, normal human dermal fibroblasts began to infiltration into the inside of scaffolds. And the scaffolds have larger pore size, cells more deeply infiltrated on scaffolds. According these results, the porosity and pore size of nanofibers have an effect on cellular migration. Therefore ultra-sonication method was appropriated to control of the pore size of chitin nanofibrous sheet. And the three dimensional chitin scaffolds might be a potential material as tissue engineering.

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