타이타늄 표면에 코팅된 epigallocatechin gallate의 사람치수세포 분화 촉진

다국어 초록 (Multilingual Abstract)

Epigallocatechin gallate (EGCG), a flavonoid which is found in green tea and red wine, is known to have an osteogenic activity. In this study, the effect of EGCG on the differentiation of human dental pulp cells (HDPCs) was investigated. The growth of HDPCs was suppressed when the cells were exposed to 60 μM EGCG during 5 days. EGCG enhanced ALP activity of HDPCs at concentrations ranging from 10 to 30 μM. Furthermore, the mRNA expression of osteocalcin (OCN) and dentin sialophosphoprotein (DSPP) in HDPCs
was enhanced by exposure to EGCG. Extracellular matrix mineralization was also promoted by EGCG even in the absence of other odontogenic factors such as ascorbic acid and dexamethasone. Therefore, it is certain that EGCG is capable of inducing odontogenicity of dental pulp cells. In the experiment of biomimetic apatite coating of titanium discs, EGCG was successfully incorporated into apatite coats and released from the surfaces into culture media.

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