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      Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase(TPS1) gene from sacccharomyces cerevisiae

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      https://www.riss.kr/link?id=E805493

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      In yeast, trehalose-6-phosphate synthase is a key enzyme for trehalose biosynthesis, encoded by the structrual gene TPS1. Trehalose affects sugar metabolism as well as osmoprotection against several environmental stresses, such as heat and desiccation. The TPS1 gene of Saccharomyces cerevisiae was engineered under the control of the CaMV 35S promoter for constitutive expression in transgenic potato plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting TPS1 transgenic potato plants exhibited various morphological phenotypes in culture tubes, ranging from normal to severely retarded growth, including dwarfish growth, yellowish lancet-shaped leaves, and aberrant root development. However, the plants recovered from these negative growth effects when grown in a soil mixture. The TPS1 transgenic potato plants showed significantly increased drought resistance. These results suggest that the production of trebalose not only affects plant development but also improves drought tolerance.
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      In yeast, trehalose-6-phosphate synthase is a key enzyme for trehalose biosynthesis, encoded by the structrual gene TPS1. Trehalose affects sugar metabolism as well as osmoprotection against several environmental stresses, such as heat and desiccation...

      In yeast, trehalose-6-phosphate synthase is a key enzyme for trehalose biosynthesis, encoded by the structrual gene TPS1. Trehalose affects sugar metabolism as well as osmoprotection against several environmental stresses, such as heat and desiccation. The TPS1 gene of Saccharomyces cerevisiae was engineered under the control of the CaMV 35S promoter for constitutive expression in transgenic potato plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting TPS1 transgenic potato plants exhibited various morphological phenotypes in culture tubes, ranging from normal to severely retarded growth, including dwarfish growth, yellowish lancet-shaped leaves, and aberrant root development. However, the plants recovered from these negative growth effects when grown in a soil mixture. The TPS1 transgenic potato plants showed significantly increased drought resistance. These results suggest that the production of trebalose not only affects plant development but also improves drought tolerance.

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      목차 (Table of Contents)

      • Materials and Methods
      • Results
      • Discussion
      • Acknowledgments
      • References
      • Materials and Methods
      • Results
      • Discussion
      • Acknowledgments
      • References
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