산약 추출물의 항산화 및 항염증에 관한 실험적 연구 = Experimental Study on the Antioxidant and Antimicrobial Properties of Dioscoreae Rhizoma

다국어 초록 (Multilingual Abstract)

Objectives : This study evaluated the effect of Dioscoreae Rhizoma on antioxidant and antimicrobial activity in vitro. Methods : In vitro experiments were made on antioxidant by DPPH radical scavenging activity, enzyme activity of SOD, protective effect about $H_2O_2$ and DNA fragmentation in RAW264.7 cells, as well as antimicrobial by Cox-2 expression in LPS-induced RAW264.7 cells. Results : 1. Dioscoreae Rhizoma's antioxidant ability was improved by DPPH radical scavenging activity. 2. Antioxidant ability of Dioscoreae Rhizoma produced good results at more than 0.025 mg/$m{\ell}$. 3. On protective effect of Dioscoreae Rhizoma about $H_2O_2$ on RAW264.7 cells, with addition of Dioscoreae Rhizoma extract the cells' survival increased compared to adding $H_2O_2$ only. 4. About revelation-control of Cox-2 at RAW264.7 cells which induce by LPS, when Dioscoreae Rhizoma's concentration was higher from 0.0125 mg/$m{\ell}$ to 1 mg/$m{\ell}$, its control activity was stronger. Thus we found Dioscoreae Rhizoma has antimicrobial activity. 5. The power of DNA fragmentation control of Dioscoreae Rhizoma was time-independent. Conclusions : From this study we conclude that Dioscoreae Rhizoma has effects on antioxidant and antimicrobial activity. However, considerable work still needs to be done studying antioxidants by concentration, as well as a variety of experiments about antimicrobial activity.

참고문헌 (Reference)

1 康秉秀, "臨床配合本草學" 永林社 106-108, 1996

2 곽규호, "六味地黃湯加山藥이 Alloxan 糖尿 白鼠의 血糖 및 血淸變化에 미치는 影響" 8 : 137-156, 1993

3 김호철, "한방약리학" 교학사 835-, 1998

4 강경아, "산화적 스트레스에 대한 생약 추출물의 항 산화활성 검색" 한국생약학회 36 (36): 159-163, 2005

5 전영준, "산약의 HeLa cell 분화에 미치는 영향과 항산화효과에 대한연구" 대한한방부인과학회 20 (20): 139-154, 2007

6 신상우, "대표적 보기약인 인삼, 당삼, 황기, 백출, 산약 물추출액의 면역조절효과 비교" 대한동의병리학회 18 (18): 1140-1146, 2004

7 장남수, "뇌 조직에서 알코올 투여에 대한 녹차 건분의 항산화 효과" 34 (34): 525-531, 2001

8 전국 한의과대학 피부외과학 교재편찬위원회, "韓醫皮膚外科學" 도서출판 선우 81-82, 2007

9 許浚, "東醫寶鑑" 南山堂 1182-, 1998

10 楊東喜, "本草備要解析" 醫聖堂 491-, 1993

11 임진석, "本經疎證" 아티전 79-, 1998

12 양정민, "山藥의 항산화 작용에 대한 단백질체 분석 연구" 대한한방부인과학회 21 (21): 108-124, 2008

13 Lee SC, "The evalution of Hepato-protective Effects of Huai-Shan-Yao (Rhizoma Dioscoreae)" 30 (30): 609-616, 2002

14 Hu K, "The cytotoxicity of methyl protodioscin against human cancer cell lines in vitro" 21 (21): 389-393, 2003

15 Papa S, "Reactive oxygen species, mitochondria, apoptosis and aging" 174 (174): 305-319, 1997

16 Borrello S, "Protective enzymes in human epidermal carcinomas and psoriasis" 276 : 338-340, 1984

17 Adelman R, "Oxidative damage to DNA; relation to species metabolic rate and life span" 85 : 2706-2708, 1998

18 Liu SF, "NF-κB activation as a pathological mechanism of septic shock and inflammation" 290 : 622-645, 2005

19 Kim MJ, "Methanol extract of Dioscoreae rhizoma inhibits pro-inflammatory cytokines and mediators in the synoviocytes of rheumatoid arthritis" 4 (4): 1489-1497, 2004

20 Tanno N, "Identification of Endogenous Gibberellins Dormant Bulbils of Chinese Yam. Diosorea opposita" 100 : 1823-1826, 1992

21 Thengchaisri N, "Hydrogen peroxide induces endothelium-dependent and -independent coronary arteriolar dilation: role of cyclooxygenase and potassium channels" 285 : 2255-2263, 2003

22 Halliwell B, "Free radicals, antioxidants and human disease: where are we now?" 119 : 598-620, 1992

23 황귀서, "Estrogen 缺乏性 骨多孔症에 미치는 山藥抽出物의 影響" 7 (7): 55-66, 2003

24 Halliwell B, "Drug antioxidant effects. A basis for drug selection?" 42 (42): 569-605, 1991

25 Cai J, "Apoptosis induced by dioscin in Hela cells" 25 (25): 193-96, 2002

26 Halliwell B, "Antioxidants in human health and disease" 16 : 33-50, 1996

27 Blois MS, "Antioxidant determination by the use of a stable free radical" 181 : 1199-1200, 1958

28 Acharyr K, "Antioxidant and nitric oxide synthase activation properties of Auricularia auricula" 42 (42): 538-540, 2004

29 Harman D, "Aging; A theory based on free radical and radical chemistry" 11 (11): 298-, 1956