5-Fluorouracil이 생쥐 콩팥소체의 미세구조에 미치는 영향 = Fine Structural Changes of the Renal Corpuscle of the Mice followint the Administration of 5-Fluorouracil

다국어 초록 (Multilingual Abstract)

The experiment was performed to study the morphological responses of the renal glomeruli of the mice after administration of 5-fluorouracil.
Healthy adult mice weighing about 25gm each were divided into normal and 5-fluorouracil treated groups. 5-fluorouracil(60mg/kg) were injected subcutaneously to the animals every other day, and animals were sacrificed at 4 days or 7days following the first injections.
Pieces of tissues were taken from renal coriticies, prefixed with 2.5% glutaraldehyde- 1.5% paraformaldehyde(0.1M Millonig's phosphate buffter, pH 7.3), and followed by post-fixation with 1% osmium tetroxide(0.1M Millonig's phosphate buffer, pH 7.3). Fixed blocks were dehydrated with alcohol and acetone, embedded in araldite mixture, cut with LKB V-ultrotome. The ultrathin sections stained with uranyl acetate and lead citrate were observed with a JEM 100CX-Ⅱ electron microscope.
In the fourth day following the first injection of 5-fluorouracil, components of the renal glomeruli of the mice are looked compact since they were filled with the widened the mesangium, and showed narrowing lumen of glomerular capillaries and of urinary spaces. The cisternae of the rough endoplasmic reticulum of the podocytes were slightly swollen, whereas flattened cistrernae of the Golgi complexes were frequently observed. And in the fourth day after the first injection of 5-fluorouracil, the compact arragement of renal golmeruli of the mice were stil observed, but morphological changes of glomeruli were generally recovered in the seventh day.
From the above results, it was concluded that the large amout of 5-fluorouracil(60mg/kg)induces slightly cytotoxic effects of the renal glomeruli, but fine structural changes occurred inthe renal glomeruli after injection of 5-fluorouracil were recovered to normal apperance within 1 week.