Preterm premature rupture of membrane (PROM) model and the regenerative effect of folic acid on amniotic membrane

다국어 초록 (Multilingual Abstract)

Abstract
Objective
To examine the effects of nicotine and folic acid on amniotic regeneration in an in-vitro PROM model.
Methods
Human Amniotic Epithelial Stem Cells (hAESCs) were isolated from the human amniotic membrane and Human Amnion Tissue Cells (hATCs) were derived from the amnion tissue membrane. Both cell groups were prepared and characterized by flow cytometry. The effects of nicotine and folic acid on cell viability at different concentration levels were examined. In addition, the protective effect of folic acid on nicotine was also studied.

Results
Stem cell markers such as OCT-3/4, SSEA-4, SOX-2, Nanog were strongly expressed in hAESCs but only weakly expressed in hATCs. In addition, CD19 and CD34 were negative but CD73, CD90, and CD105 were positive in both cell groups. hAESCs showed a strong expression of CD324 whereas it barely expressed CD349. However, hATCs remained a high expression of CD349 with a weak level of CD324. Nicotine treatment significantly decreased the cell viabilities with the ranges from 2 mM to 5 mM (p<0.01) for hAESCs and from 3 mM to 5 mM (p<0.05) for hATCs. When applying folic acid on hAESCs, the cell viability increased significantly at a concentration of 100 nM (p<0.05), but it had no such effect on hATCs. Moreover, hAESCs treated with folic acid showed a protective result on nicotine treatment at the concentration of 100 nM of folic acid (p<0.05). Likewise, the similar pattern appeared in hATCs with the concentration of 100 nM of folic acid (p<0.05).

Conclusion
hAESCs and hATCs isolated and cultured from amniotic membrane showed similar characteristics except for slight differences in expression levels of OCT- 3/4, SSEA-4, SOX-2, Nanog. Cell viabilities significantly decreased with nicotine treatment in both cell groups, but decreased less with co-incubation with folic acid. Therefore, we found that folic acid has regenerative and protective effects on amniotic membrane.

목차 (Table of Contents)

• Table of Contents
• 1) Abstract 2
• 2) Introduction 4
• 3) Materials and Methods 5
• 4) Results 7
• 5) Discussion 14
• 6) Conclusion 15
• 7) References 16