흑삼의 신생혈관 억제활성에 대한 연구

다국어 초록 (Multilingual Abstract)

Objectives: This study was performed to investigate the influence of black ginseng radix extracts (BG) and ginsenoside Rg3, Rg5 on basic fibroblast growth factor (bFGF) induced proliferation, migration and capillary tubule-like formation of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were cultured with BG and ginsenoside Rg3, Rg5 at different concentrations (60, 125, 250, 500, 1,000 ㎍/㎖) for 2 day in the presence of bFGF, respectively. XTT was used to detect the proliferation. Migration and tube formations were examined to detect the antiangiogenesis. Also, the chick embryo chorioallantoic membrane (CAM) assay was performed to detect the antiangiogenesis. Results: BG and ginsenoside Rg3, Rg5 significantly inhibited bFGF-induced endothelial cell proliferation and migration in a dose-dependent manner. Tube formation in bFGF-induced HUVECs were suppressed by BG and ginsenoside Rg3, Rg5. Moreover, BG and ginsenoside Rg3, Rg5 (30-50 ㎍/egg) inhibited new blood vessel formation on the growing CAM. Conclusions: Based on the present results, it can be suggested that BG has a potential chemopreventive agent via antiangiogenesis.

참고문헌 (Reference)

1 이지현, "흑삼의 제조 및 항암효과" 대한동의병리학회 20 (20): 951-956, 2006

2 김효진, "흑삼 제조과정 중 증포 횟수에 따른 에탄올 추출물의 항산화 활성" 한국식품영양과학회 40 (40): 156-162, 2011

3 이미라, "포도주스 침지 제조 흑삼의 Ginsenoside Rg3 함량 변화와 Acetylcholinesterase 억제효과" 고려인삼학회 33 (33): 349-354, 2009

4 송규용, "정상 식이 수컷 흰쥐에서 흑삼이 체중 및 지질에 미치는 영향" 대한약학회 50 (50): 381-385, 2006

5 김의검, "신공법에 의한 흑삼의 제조 및 항암활성" 대한본초학회 23 (23): 85-92, 2008

6 김준식, "식물 추출물의 혈관신생 억제 효능 검색" 한국생약학회 37 (37): 253-257, 2006

7 허정은, "수종의 한약재에서 신생혈관형성 활성 검색 및 기전 연구" 대한침구의학회 24 (24): 23-32, 2007

8 Bergers G, "Tumorigenesis and the angiogenic switch" 3 : 401-410., 2002

9 Folkman J, "Tumor angiogenesis：therapeutic implications" 285 : 1182-1186, 1971

10 Chen MW, "The inhibition of 20(R)-ginsenoside Rg3 on the expressions of angiogenesis factors proteins in human lung adenocarcinoma cell line A549 and HUVEC304 cell" 30 (30): 357-360, 2005

11 Lee SI, "The history of Korean ginseng 2nd" Samhwa press 166-, 1980

12 Yue PY, "The angiosuppressive effects of 20(R)-ginsenoside Rg3" 72 (72): 437-445, 2006

13 Ae-Jung Kim, "The Chemopreventive Potential and Anti-inflammatory Activities of Korean Black Ginseng in Colon26-M3.1 Carcinoma Cells and Macrophages" 한국응용생명화학회 53 (53): 101-105, 2010

14 Yu LC, "Stability of angiogenic agents, ginsenoside Rg1 and Re, isolated from Panax ginseng：in vitro and in vivo studies" 328 (328): 168-176, 2007

15 Ha YW, "Preparative isolation of four ginsenosides from Korean red ginseng (steam-treated Panax ginseng C. A. Meyer), by high-speed countercurrent chromatography coupled with evaporative light scattering detection" 1151 (1151): 37-44, 2007

16 Plate KH, "Molecular mechanisms of developmental and tumor angiogenesis" 4 : 207-218, 1994

17 Boyd D, "Invasion and metastasis" 15 (15): 77-89, 2006

18 Grant DS, "Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo" 153 : 614-625, 1992

19 Nam NH, "Inhibitory effects of Vietnamese medicinal plants on tubelike formation of human umbilical venous cells" 17 : 107-111, 2003

20 Jost LM, "Improved short- and long-term XTT-based colorimetric cellular cytotoxicity assay for melanoma and other tumor cells" 147 : 153-165, 1992

21 Liu L, "Enzymatic preparation of 20(S,R)-protopanaxadiol by transformation of 20(S,R)-Rg3 from black ginseng" 71 (71): 1514-1520, 2010

22 Yue PY, "Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg(1) in vivo and in vitro" 8 (8): 205-216, 2005

23 Reynolds AR, "Elevated Flk1 (Vascular Endothelial Growth Factor Receptor 2) Signaling Mediates Enhanced Angiogenesis in ß3-Integrin– Deficient Mice" 64 : 8643-8650, 2004

24 Mi-Ra Lee, "Effect of Black Ginseng on Memory Improvement in the Amnesic Mice Induced by Scopolamine" 고려인삼학회 34 (34): 51-58, 2010

25 Bai-Shen Sun, "Determination of 11 Ginsenosides in Black Ginseng Developed from Panax ginseng by High Performance Liquid Chromatography" 한국식품과학회 18 (18): 561-564, 2009

26 Kim Y, "Deoxypodophyllotoxin; the cytotoxic and antiangiogenic component from Pulsatilla koreana" 68 (68): 271-274, 2003

27 Nguyen Huu Tung, "Dammarane-type Saponins from the Black Ginseng" 대한화학회 31 (31): 3423-3426, 2010

28 Jaffe EA, "Culture of human endothelial cells derived from umbilical veins.Identification by morphologic and immunologic criteria" 52 : 2745-2756, 1973

29 Keum YS, "Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng" ELSEVIER IRELAND LTD 150 : 41-48, 2000

30 Lee KH, "Anti-angiogenic effect of the see extract of Benincasa hipida Cogniaux" 97 : 509-513, 2005

31 Song YS, "Anti-acngiogenic, antioxidant and xanthine oxidase inhibition activities of the mushroom Phellinus linteus" 88 : 113-116, 2003

32 Kim LS, "Angiostatin and Endostatin：Endogenous Inhibitors of Tumor Growth" 19 (19): 181-190, 2000

33 Griffioen AW, "Angiogenesis：potentials for pharmacologic intervention in the treatment of cancer, cardiovascular diseases, and chronic inflammation" 52 (52): 237-268, 2000

34 Carmeliet P, "Angiogenesis in cancer and other diseases" 407 : 249-257, 2000

35 Folkman J, "Angiogenesis and apoptosis" 3 : 159-167, 2003

36 Folkman J, "Angiogenesis" 267 : 10931-10934, 1992