肝吸蟲症에 있어서 血淸 및 濾紙吸着血淸을 이용한 酵素免疫測定法 (ELISA)에 관한 比較硏究 = Comparative Studies on the Immunodiagnosis of Clonorchiasis by Means of Micro-ELISA Using Sera and Blood Collected on Filter Paper

다국어 초록 (Multilingual Abstract)

To observe the usefulness of minute amount of blood obtained by the filter paper technique for serodiagnosis of clonorchiasis sinensis, a study was performed with the subjected cases of 142 egg positive cases and O negative controls by the immunodiagnostic method of ELISA. The absorbance values using the filter paper by ELISA were compared with the absorbance values using sera, and also with the E.P.G.(eggs per gram of feces) and the diameter of wheal size by intradermal test. The antigen used was saline extract of crude Clonorchis sinensis adult worm with the protein concentration of 11.3㎍/ml. The positive threshold of the absorbance value was 0.5 at 488nm.
The results obtained were as follows:
1. Comparing the sensitivity and the specificity between the filter paper technique and the method using sera by ELISA, 121 out of 140 egg positive cases were positive(sensitivity, 88.4%) and 69 out of 70 controls were negative(specificity, 98. 6%) when the blood collected on the filter paper were used; and 132 out of 140 egg positive cases were positive (sensitivity, 93.0%) and 67 out of 70 control were negative(specificity, 95.7%) when sera were used. Thus the specificity by the filter paper method was similar to that by the sera although the sensitivity was lower.
2. The relation of the absorbance values between the blood collected on the filter paper (y-axis) and sera(x-axis) made the regression equation: y=0. 473x+0.248, and showed relatively high correlation (r=0. 739, n=210, p<0. 01).
3. The relation of the absorbance values between the blood collected on the filter paper (y-axis) and the number of eggs(Log E.P.G./100) made the regression equation: y=0. 254 x+0.654, and showed some correlation(r=O. 541, n=140, p<0.01). And the mean absorbance values of the four classified groups were increased gradually with the intensity of the infection by E.P.G.: light infection with 1~999 E.P.G., moderate infection with 1,000-9,999 E.P.G., heavy infection with 10,000~.29,999 E.P.G. and very heavy infection with the E.P.G. of 30,000 or over.
4. The relation of the absorbance values between the blood collected on the filter paper (x-axis) and the diameter of the wheal by intradermal test(y-axis) made the regression equation : y=0. 087x+1.169, and showed low correlation (r=o. 192, n=140, p<0.01).
5. The absorbance values of the blood collected on the filter paper and those of sera and the diameter of wheal by intradermal test of the egg positive cases were age-independent.
This study showed that the blood collected on the filter Gaper technique was applicable for the diagnosis of clonorchiasis by the method of ELISA.