Orientia tsutsugamushi감염 시 유발되는 마우스의 염증 반응 = Inflammatory Response of Mouse Induced by Orientia tsutsugamushi Infection

다국어 초록 (Multilingual Abstract)

Early host defense mechanisms were examined in resistant (BALB/C) and susceptible (C3H/HeN) mice after intraperitoneal inoculation with Orientia tsutsugamushi strain Gilliam. In this study, I used semiquantitative reverse transcription-PCR to examine chemokine gene expression in resistant and susceptible mice following infection with strain Gilliam. The mRNAS for macrophage inflammatory protein 1 α/β(MIP- α/β ), monocyte chemoattractant protein 1 (MCP-1), gamma-interferon-inducible protein 10 (IP-10), tumor necrosis factor alpha (TNF- α), and RANTES were detected in susceptible mice. Peak expression of these chemokines was observed between 5 and 6 days after infection. However, gene expression of MIP-1 α/β , MIP-2, MCP-1, and TNF-α was not detected in resistant mice. The production of TNF-α is well correlated with the lethal infection model. Identification of various cytokines and chemokines changed in amount during infection provides important information relevant to unraveling the host defense mechanisms for natural resistance to infection.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 실험 동물
• 리켓치아 배양 및 마우스 감염
• 복강 대식세포의 분리 및 RNA 추출
• 리켓치아 감염에 대한 염증 반응의 측정
• Semiquantitative RT-PCR
• 결과
• 고찰