Noncanonical amino acids : methods and protocols

목차 (Table of Contents)

• CONTENTS
• Preface = v
• Contributors = ix
• PART Ⅰ ENGENEERING IN THE TEST TUBE
• 1 Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates / Robyn M. Barfield ; David Rabuka = 3
• 2 Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis / Yoshihiko Iwane ; Takayuki Katoh ; Yuki Goto ; Hiroaki Suga = 17
• 3 Cell-free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains / Eiko Seki ; Tatsuo Yanagisawa ; Shigeyuki Yokoyama = 49
• 4 Tub-tag Labeling : Chemoenzymatic Incorporation of Unnatural Amino Acids / Jonas Helma ; Heinrich Leonhardt ; Christian P.R. Hackenberger ; Dominik Schumacher = 67
• PART Ⅱ ENGEERING IN PROKARYOTES
• 5 Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids / Moritz J. Schmidt ; Daniel Summerer = 97
• 6 Genetic Code Expansion in Enteric Bacterial Pathogens / Huangtao Zheng ; Shixian Lin ; Peng R. Chen = 113
• 7 Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins / Ying Ma ; Martino L. Di Salvo ; Nediljko Budisa = 127
• 8 Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering / Mark B. van Eldijk ; Jan C.M. van Hest = 137
• 9 Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene / Jeffery M. Tharp ; Wenshe R. Liu = 147
• 10 Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439 / Jingxuan He ; Charles E. Melancon III = 155
• 11 Expression and Purification of Site-Specifically Lysine-Acetylated and Natively-Folded Proteins for Biophysical Investigations / Michael Lammers = 169
• 12 Site-Specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code / Xiang Li ; Chang C. Liu = 191
• 13 Site-Specific Protein Labeling with Tetrazine Amino Acids / Robert J. Blizzard ; True E. Gibson ; Ryan A. Mehl = 201
• PART Ⅲ ENGINEERING IN EUKARYOTES
• 14 Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers / Lisa Seidel ; Irene Coin = 221
• 15 Generation of Stable Amber Suppression Cell Lines / Simon J. Elsässer = 237
• 16 Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo / Christian Hoffmann ; Heinz Neumann ; Petra Neumann-Staubitz = 247
• 17 Genetically Encoding Unnatural Amino Acids in Neurons in Vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins / Ji-Yong Kang ; Daichi Kawaguchi ; Lei Wang = 263
• 18 Genetic Code Expansion-and Click Chemistry-Based Site-Specific Protein Labelling For Intracellular DNA-PAINT Imaging / Ivana Nikić-Spiegel = 279
• 19 MultiBacTAG- Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells / Christine Koehler ; Edward A. Lemke = 297
• 20 Production and Chemoselective Modification of Adeno-Associated Virus Site-Specifically Incorporating an Unnatural Amino Acid Residue into Its Capsid / Rachel E. Kelemen ; Sarah B. Erickson ; Abhishek Chatterjee = 313
• 21 Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis / Simone Brand ; Yao-Wen Wu = 327
• 22 Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins / Eszter Kozma ; Giulia Paci ; Gemma Estrada Girona ; Edward A. Lemke ; Péter Kele = 337
• 23 Site-Specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction / Mathis Baalmann ; Marcel Best ; Richard Wombacher = 365
• 24 Genetic Encoding of Unnatural Amino Acids in C. elegans / Lloyd Davis ; Sebastian Greiss = 389
• Index = 409