RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      KCI등재 SCI SCIE SCOPUS

      In vivo Tracking of Mesenchymal Stem Cells Labeled with a Novel Chitosan-coated Superparamagnetic Iron Oxide Nanoparticles using 3.0T MRI

      한글로보기

      https://www.riss.kr/link?id=A104779034

      • 0

        상세조회
      • 0

        다운로드
      서지정보 열기
      • 내보내기
      • 내책장담기
      • 공유하기
      • 오류접수

      부가정보

      다국어 초록 (Multilingual Abstract)

      This study aimed to characterize and MRI track the mesenchymal stem cells labeled with chitosan-coated superparamagnetic iron oxide (Chitosan-SPIO). Chitosan-SPIO was synthesized from a mixture of FeCl2 and FeCl3. The human bone marrow derived mesenchymal stem cells (hBM-MSC) were labeled with 50 mg Fe/mL chitosan-SPIO and Resovist. The labeling efficiency was assessed by iron content, Prussian blue staining, electron microscopy and in vitro MR imaging. The labeled cells were also analyzed for cytotoxicity, phenotype and differentiation potential. Electron microscopic observations and Prussian blue staining revealed 100% of cells were labeled with iron particles. MR imaging was able to detect the labeled MSC successfully.
      Chitosan-SPIO did not show any cytotoxicity up to 200 mg Fe/mL concentration. The labeled stem cells did not exhibit any significant alterations in the surface markers expression or adipo/osteo/chondrogenic differentiation potential when compared to unlabeled control cells. After contralateral injection into rabbit ischemic brain, the iron labeled stem cells were tracked by periodical in vivo MR images. The migration of cells was also confirmed by histological studies. The novel chitosan-SPIO enables to label and track MSC for in vivo MRI without cellular alteration.
      번역하기

      This study aimed to characterize and MRI track the mesenchymal stem cells labeled with chitosan-coated superparamagnetic iron oxide (Chitosan-SPIO). Chitosan-SPIO was synthesized from a mixture of FeCl2 and FeCl3. The human bone marrow derived mesench...

      This study aimed to characterize and MRI track the mesenchymal stem cells labeled with chitosan-coated superparamagnetic iron oxide (Chitosan-SPIO). Chitosan-SPIO was synthesized from a mixture of FeCl2 and FeCl3. The human bone marrow derived mesenchymal stem cells (hBM-MSC) were labeled with 50 mg Fe/mL chitosan-SPIO and Resovist. The labeling efficiency was assessed by iron content, Prussian blue staining, electron microscopy and in vitro MR imaging. The labeled cells were also analyzed for cytotoxicity, phenotype and differentiation potential. Electron microscopic observations and Prussian blue staining revealed 100% of cells were labeled with iron particles. MR imaging was able to detect the labeled MSC successfully.
      Chitosan-SPIO did not show any cytotoxicity up to 200 mg Fe/mL concentration. The labeled stem cells did not exhibit any significant alterations in the surface markers expression or adipo/osteo/chondrogenic differentiation potential when compared to unlabeled control cells. After contralateral injection into rabbit ischemic brain, the iron labeled stem cells were tracked by periodical in vivo MR images. The migration of cells was also confirmed by histological studies. The novel chitosan-SPIO enables to label and track MSC for in vivo MRI without cellular alteration.

      더보기

      참고문헌 (Reference)

      1 Newcomb CD, "Timing of cord blood treatment after experimental stroke determines therapeutic efficacy" 15 : 213-223, 2006

      2 Kim EH, "Synthesis of ferrofluid with magnetic nanoparticles by sonochemical method for MRI contrast agent" 289 : 328-330, 2005

      3 Lee HS, "Synthesis of MRI contrast agent by coating superparamagnetic iron oxide with chitosan" 41 : 4102-4104, 2005

      4 Wang YX, "Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imaging" 11 : 2319-2331, 2001

      5 Stauer BE, "Stem cell therapy in perspective" 107 : 929-934, 2003

      6 Deans RJ, "Mesenchymal stem cells: biology and potential clinical uses" 28 : 875-884, 2000

      7 Harris LA, "Magnetic nanoparticle dispersions stabilized with triblock copolymers" 15 : 1367-1377, 2003

      8 Arbab AS, "Labeling of cells with ferumoxides. Protamine sulfate complexes does not inhibit function or differentiation capacity of hematopoitic or mesenchymal stem cells" 18 : 553-559, 2005

      9 Werner N, "Intravenous transfusion of endothelial progenitor cells reduces neointima formation after vascular injury" 93 : 17-24, 2003

      10 Ittrich H, "In vivo magnetic resonance imaging of iron oxide labeled arterially injected mesenchymal stem cells in kidney of rats with acute ischemic kidney injury: detection and monitoring at 3T" 25 : 1179-1191, 2007

      1 Newcomb CD, "Timing of cord blood treatment after experimental stroke determines therapeutic efficacy" 15 : 213-223, 2006

      2 Kim EH, "Synthesis of ferrofluid with magnetic nanoparticles by sonochemical method for MRI contrast agent" 289 : 328-330, 2005

      3 Lee HS, "Synthesis of MRI contrast agent by coating superparamagnetic iron oxide with chitosan" 41 : 4102-4104, 2005

      4 Wang YX, "Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imaging" 11 : 2319-2331, 2001

      5 Stauer BE, "Stem cell therapy in perspective" 107 : 929-934, 2003

      6 Deans RJ, "Mesenchymal stem cells: biology and potential clinical uses" 28 : 875-884, 2000

      7 Harris LA, "Magnetic nanoparticle dispersions stabilized with triblock copolymers" 15 : 1367-1377, 2003

      8 Arbab AS, "Labeling of cells with ferumoxides. Protamine sulfate complexes does not inhibit function or differentiation capacity of hematopoitic or mesenchymal stem cells" 18 : 553-559, 2005

      9 Werner N, "Intravenous transfusion of endothelial progenitor cells reduces neointima formation after vascular injury" 93 : 17-24, 2003

      10 Ittrich H, "In vivo magnetic resonance imaging of iron oxide labeled arterially injected mesenchymal stem cells in kidney of rats with acute ischemic kidney injury: detection and monitoring at 3T" 25 : 1179-1191, 2007

      11 Kostura L, "Feridex labeling of mesenchymal stem cells inhibits chondrogenesis but not adipogenesis or osteogenesis" 17 : 513-517, 2004

      12 Arbab AS, "Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI" 104 : 1217-1223, 2004

      13 Cheng SL, "Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone" 134 : 277-286, 1994

      14 Berry CC, "Dextran and albumin derivatised iron oxide nanoparticles: influence on fibroblasts in vitro" 24 : 4551-4557, 2003

      15 Bulte JW, "Chondrogenic differentiation of mesenchymal stem cells is inhibited after magnetic labeling with ferumoxides" 104 : 3410-3413, 2004

      16 Mackay AM, "Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow" 4 : 415-428, 1998

      17 Sinha VR, "Chitosan microspheres as a potential carrier for drugs" 274 : 1-33, 2004

      18 Arbab AS, "Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging" 229 : 838-846, 2003

      19 Bowen CV, "Application of the static dephasing regime theory to superparamagnetic iron-oxide loaded cells" 48 : 52-61, 2002

      20 Taguchi A, "Administration of CD34+ cells after stroke enhances neurogenesis via angiogenesis in a mouse model" 114 : 330-338, 2004

      더보기

      분석정보

      View

      상세정보조회

      0

      Usage

      원문다운로드

      0

      대출신청

      0

      복사신청

      0

      EDDS신청

      0

      동일 주제 내 활용도 TOP

      더보기

      주제

      연도별 연구동향

      연도별 활용동향

      연관논문

      연구자 네트워크맵

      공동연구자 (7)

      유사연구자 (20) 활용도상위20명

      인용정보 인용지수 설명보기

      학술지 이력

      학술지 이력
      연월일 이력구분 이력상세 등재구분
      2023 평가예정 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
      2020-01-01 평가 등재학술지 유지 (해외등재 학술지 평가) KCI등재
      2011-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2009-01-01 평가 등재학술지 유지 (등재유지) KCI등재
      2005-01-01 평가 SCI 등재 (등재유지) KCI등재
      2002-01-01 평가 등재학술지 선정 (등재후보2차) KCI등재
      1999-07-01 평가 등재후보학술지 선정 (신규평가) KCI등재후보
      더보기

      학술지 인용정보

      학술지 인용정보
      기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
      2016 1.48 0.37 1.06
      KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
      0.85 0.75 0.691 0.11
      더보기

      이 자료와 함께 이용한 RISS 자료

      나만을 위한 추천자료

      해외이동버튼