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다국어 초록 (Multilingual Abstract)

BACKGROUND: The formation of an inhibitory inflammatory microenvironment after spinal cord injury (SCI) remains a great challenge for nerve regeneration. The poor local microenvironment exacerbates nerve cell death; therefore, the reconstruction of a favorable microenvironment through small-molecule drugs is a promising strategy for promoting nerve regeneration.
METHODS: In the present study, we synthesized curcumin-loaded micelle nanoparticles (Cur-NPs) to increase curcumin bioavailability and analyzed the physical and chemical properties of Cur-NPs by characterization experiments. We established an in vivo SCI model in rats and examined the ability of hind limb motor recovery using Basso–Beattie– Bresnahan scoring and hind limb trajectory assays. We also analyzed neural regeneration after SCI using immunofluorescence staining.
RESULTS: The nanoparticles achieved the intelligent responsive release of curcumin while improving curcumin bioavailability. Most importantly, the released curcumin attenuated local inflammation by modulating the polarization of macrophages from an M1 pro-inflammatory phenotype to an M2 anti-inflammatory phenotype. M2-type macrophages can promote cell differentiation, proliferation, matrix secretion, and reorganization by secreting or expressing pro-repair cytokines to reduce the inflammatory response. The enhanced inflammatory microenvironment supported neuronal regeneration, nerve remyelination, and reduced scar formation. These effects facilitated functional repair in rats, mainly in the form of improved hindlimb movements.
CONCLUSION: Here, we synthesized pH/temperature dual-sensitive Cur-NPs. While improving the bioavailability of the drug, they were also able to achieve a smart responsive release in the inflammatory microenvironment that develops after SCI. The Cur-NPs promoted the regeneration and functional recovery of nerves after SCI through anti-inflammatory effects, providing a promising strategy for the repair of SCIs.

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참고문헌 (Reference)

1 Kim Do-Hun ; Cho Hyun-Ju ; Park Chul-Yong ; 조명수 ; Kim Dong-Wook, "Transplantation of PSA-NCAM-Positive Neural Precursors from Human Embryonic Stem Cells Promotes Functional Recovery in an Animal Model of Spinal Cord Injury" 한국조직공학과 재생의학회 19 (19): 1349-1358, 2022

2 Salewski RP, "Transplantation of Induced pluripotent stem cellderived neural stem cells mediate functional recovery following thoracic spinal cord injury through remyelination of axons" 4 : 743-754, 2015

3 Sanivarapu R, "The potential of curcumin in treatment of spinal cord injury" 2016 : 9468193-, 2016

4 Norenberg MD, "The pathology of human spinal cord injury : defining the problems" 21 : 429-440, 2004

5 Milich LM, "The origin, fate, and contribution of macrophages to spinal cord injury pathology" 137 : 785-797, 2019

6 Dunster JL, "The macrophage and its role in inflammation and tissue repair : mathematical and systems biology approaches" 8 : 87-99, 2016

7 Li W, "The fine-tuning of thermosensitive and degradable polymer micelles for enhancing intracellular uptake and drug release in tumors" 32 : 3832-3844, 2011

8 Bloom O, "Systemic inflammation in traumatic spinal cord injury" 325 : 113143-, 2020

9 Liu X, "Selenium nanoparticles derived from Proteus mirabilis YC801 alleviate oxidative stress and inflammatory response to promote nerve repair in rats with spinal cord injury" 9 : rbac042-, 2022

10 Stenudd M, "Role of endogenous neural stem cells in spinal cord injury and repair" 72 : 235-237, 2015