Evaluation of Aflatoxin B1 Biosynthesis in A. flavus Isolates from Central India and Identification of Atoxigenic Isolates

다국어 초록 (Multilingual Abstract)

Present study describes the effect of temperatureand pH on biosynthesis of aflatoxin B1 (AFB1) to identifyatoxigenic (aflatoxin non-producer) isolates of Aspergillusflavus. An indigenous detection method for AFB1 wasdeveloped using tandem mass spectrometric method.
Detection of AFB1 was carried out in positive polarityusing triple quadrapole mass spectrometer with electrosprayionization interface in Multiple Reaction Monitoring(MRM) mode. A total of four A. flavus isolates wereselected for screening of AFB1 biosynthesis at pH 4.0, 5.5,7.0, and 8.5 at 27°C. Highest AFB1 biosynthesis inMTCC11866 was found at pH 6.5 while in MTCC9367 itwas at pH 7.0. On screening AFB1 biosynthesis in A. flavusculture at various temperatures a significant suppression inAFB1 biosynthesis was found at 37°C in comparison to 24and 27°C in MTCC11866 and MTCC9367. AFB1 was notdetected in other two A. flavus isolates MTCC11580 andMTCC11588 at any temperature and pH tested. AFB1nonproducing isolates, MTCC11580 and MTCC11588could be used as potent biocontrol agent. Additionally,present standardized method for AFB1 detection may findits application in qualitative and quantitative analysis ofAFB1 contamination in food crop and other products.

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