腹腔渗出細胞를 移入받은 마우스와 햄스터에서 肝吸蟲에 대한 免疫應答의 比較 = Comparison of Immune Responses to Clonorchis sinensis Between Mice and Hamsters Given Pertioneal Exuate Cells

국문 초록 (Abstract)

肝吸蟲의 代謝産物로 마우스와 golden햄스터를 感作하여 4日 經過한 後 이들 動物에서 腹腔渗出細胞를 採集하여 正常마우스와 햄스터의 腹腔內에 注入한 다음 challenge 感染시켜 肝吸蟲에 對한 BALB/c마우스와 golden햄스터의 免疫應答을 比較하였다.
마우스와 햄스터는 各各 2群으로 나누어 第Ⅰ群 마우스는 adjuvant와 肝吸蟲 代謝産物의 混合物을 腹腔內에 注入하였고 第Ⅱ群 마우스는 同混合物을 footpad에 皮下注射하였으며 第Ⅲ群 햄스터는 同混合物을 腹腔內, 第Ⅳ群 햄스터는 同混合物을 footpad의 皮下에 注射하였다. 4日 經過한 後 마우스와 햄스터를 모두 屠殺하여 腹腔渗出細胞를 採集하였다.
recipient마우스는 2群으로 나누어 第1群은 第Ⅰ群 마우스의 腹腔渗出細胞를 第2群에는 第Ⅱ群 마우스의 腹腔渗出細胞를 各各 腹腔內에 注入하였다. recipient 햄스터도 2群으로 나누어 第3群은 第Ⅲ群 햄스터의 腹腔渗出細胞를, 第4群에는 第Ⅳ群햄스터의 腹腔渗出細胞를 腹腔內에 注入하였으며 第5群은 마우스와 햄스터를 對照群으로 두었다.
腹腔渗出細胞를 移入한 4日後에 마우스와 햄스터에 肝吸蟲 被襄幼蟲을 經口的으로 challenge 感染시켜 隔日로 EpG로 計測하였으며 約 40日 後에 모두 屠殺하여 마우스와 햄스터에서의 EpG變動狀, worm burden 및 脾臟當 plague形成細胞數等을 比較하였다.
마우스에서의 EpG는 challenge感染後 23日에 처음 나타났고 29日과 31日에 最大値를 나타내었는데 比하여 햄스터에서는 18日에 처음 나타났고 그後 서서히 增加되었으며 39日에서 43日 사이에 最大値를 나타내었다.
肝吸蟲의 蟲體回收率과 plaque形成細胞數는 마우스와 햄스터 間에 有意的 差異를 認定할 수 없었다.
以上의 成績으로 미루어보아 肝吸蟲의 實驗에는 마우스보다 햄스터가 적절한 것으로 나타났다.

다국어 초록 (Multilingual Abstract)

This study was performed to compare the role of peritoneal exudate cells between recipient BALB/c mice and golden hamsters. The peritoneal exudate cells obtained from donor mice and golden hamsters were sensitized with the admixture of the metabolic products of Clonorchis sinensis and incomplete adjuvant. The immunologic reactions in mice were compared with those in hamsters. The donor mice and hamsters were divided into 2 groups. Group Ⅰ mice were injected intraperitoneally with the admixture, and Group Ⅱ mice into the footpads. Similarly, Group Ⅲ hamsters were injected intraperitoneally and Group Ⅳ hamsters into the footpads.
Four days after sensitization, the mice were killed by neck fracture and the hamsters by deep anesthesia, and then the peritoneal exudate cells were collected from both animals.
Recipient mice and hamsters were divided into 2 groups. The mice of Group 1 were injected intraperitoneally with peritoneal exudate cells from Group Ⅰ donor mice and those of Group 2 were injected with the cells from Group Ⅱ mice. At the same time, the hamsters of Group 3 were injected intraperitoneally with peritoneal exudate cells from Group Ⅲ donor hamsters and those of Group 4 were injected with the cells from Group Ⅳ hamsters. The Group 5 mice and hamsters served as controls.
Four days after the injection of donor's peritoneal exudate cells, recipient mice were challenged orally with 20, and hamsters with 50 metacercariae, and Eggs per Gram(EpG) of fecal samples were counted from 15 to 43 days after challenge.
Mice and hamsters were killed about 40 days after challenge, and the transfer of immunity to the recipients was estimated by significant differences in EpG, mean worm burdens, and immune cells per spleen between experimental and control groups, and between mice and hamsters.
The experiments revealed that the EpG of mice appeared for the first time in 23 days, then reached the peak between 29 and 31 days after challenge, whereas, the EpG of hamsters appeared in 18 days and increased progressively, then reached the peak between 39 and 3 days. However, there were no significant differences in the worm burdens and the number of plaque forming cells between mice and hamsters.
It is likely in the present study that the golden hamster is a more appropriate animal in the transfer of immunity against C. sinensis than the mouse.