Phaffia rhodozyma 변이주를 이용한 Astaxanthin 생산에 관한 연구 = Studies on Production of Astaxanthin using Phaffia rhodozyma Mutant

다국어 초록 (Multilingual Abstract)

Phaffia rhodozyma IFO 1012901 was treated with mutagenic agent NTG(N-methly-N'-nitro-N-nitrosoguanine) and mutant strain Phaffia rhodozyma MG5150 was isolated. Phaffia rhodozyma MG 5150 showed 3.7 times higher in astaxanthin production than that of parental strain. In increasing the high production of astaxanthin from Phaffia rhodozyma MG5150, the effect of various carbon sources, nitrogen sources, cationic metal ions, the initial pH of the medium and agitation speed were investigated. The most effective carbon and nitrogen sources were found to be glucose 15% and (NH_(4))_(2)SO_(4) 5% respectively. None of the cationic metal ions examined had any detectable stimulating effect on astaxanthin production except Zn^(+2) ion. The initial optimum pH for astaxanthin production ranged from 4.5-5.0 and agitation speed was most effective at 250 rpm. In the jar fermentor experiments under optimal culture conditions, the accumulation of astaxanthin reached about 5.78㎎/㎖ after 96 hours of cultivation and the level decreased for a further cultivation.