Induction of autophagy by oleifolioside A in HCT-116 human colorectal cancer cells

다국어 초록 (Multilingual Abstract)

In current study, we addressed the anti-cancer effect of oleifolioside A and its mechanism on the regulation of cell death in HCT-116 human colorectal cancer cells. Oleifolioside A inhibited HCT-116 cell proliferation and caused apoptosis associated with sequential activation of caspases 8 and 3, followed by PARP cleavage. Moreover, anti-LC3-positive granules and the increased LC3-II level were observed in HCT-116 cells treated with oleifolioside A, which is the specific characteristics of autophagy. Treatment of autophagy inhibiors, 3-MA and Wort, markedly accelerated the cell death by oleifolioside A and, furthermore, knockdown of Beclin-1 and Atg7 using shRNA increased oleifolioside A-induced apoptosis, suggesting a cytoprotective function of autophagy against oleifolioside A-triggered apoptosis. Treatment of HCT-116 cells with oleifolioside A time-dependently activated extracellular signal-regulated kinase (ERK). Oleifolioside A-induced autophagy was dramatically inhibited by pretreatment with an ERK inhibitor, U0126, which resulted in a marked reduction in cell viability. These findings indicate that oleifolioside A induce autophagy through ERK activation in HCT-116 cells and that autophagy suppression enhances apoptosis induced by oleifolioside A.

참고문헌 (Reference)

1 Sui X, "p38 and JNK MAPK pathways control the balance of apoptosis and autophagy in response to chemotherapeutic agents" 344 : 174-179, 2014

2 Bae K, "The Medicinal Plants of Korea" Kyo-Hak Publishing Co Ltd 2000

3 Jo C, "Sulforaphane induces autophagy through ERK activation in neuronal cells" 588 : 3081-3088, 2014

4 Fan XJ, "Salidroside induces apoptosis and autophagy in human colorectal cancer cells through inhibition of PI3K/Akt/mTOR pathway" 36 : 3559-3567, 2016

5 Kim ES, "Protective activity of Dendropanax morbifera against cisplatin-induced acute kidney injury" 40 : 1-12, 2015

6 Akram M, "Potent anti-inflammatory and analgesic actions of the chloroform extract of dendropanax morbifera mediated by the Nrf2/HO-1 pathway" 39 : 728-736, 2016

7 Kang MJ, "Polyacetylene from Dendropanax morbifera alleviates diet-induced obesity and hepatic steatosis by activating AMPK signaling pathway" 9 : e537-, 2018

8 Zhang X, "Plant natural compounds : targeting pathways of autophagy as anti-cancer therapeutic agents" 45 : 466-476, 2012

9 Wen J, "Orexin A induces autophagy in HCT-116human colon cancer cells through the ERK signaling pathway" 37 : 126-132, 2016

10 Yu HY, "Oleifolioside A, a new active compound, attenuates LPS-stimulated iNOS and COX-2 expression through the downregulation of NF-κB and MAPK activities in RAW 2647macrophages" 2012 : e637512-, 2012

11 Yu HY, "Oleifolioside A mediates caspase-independent human cervical carcinoma HeLa cell apoptosis involving nuclear relocation of mitochondrial apoptogenic factors AIF and EndoG" 60 : 5400-5406, 2012

12 Zhu M, "Myricetin induces apoptosis and autophagy by inhibiting PI3K/Akt/mTOR signalling in human colon cancer cells" 20 : 209-, 2020

13 Rahman A, "Molecular insights into the multifunctional role of natural compounds : autophagy modulation and cancer prevention" 8 : 517-, 2020

14 An HK, "Mimuloneinduced autophagy through p53-mediated AMPK/mTOR pathway increases caspase-mediated apoptotic cell death in A549 human lung cancer cells" 9 : e114607-, 2014

15 Boatright KM, "Mechanisms of caspase activation" 15 : 725-731, 2003

16 Zhou YY, "MAPK/JNK signalling : a potential autophagy regulation pathway" 35 : e00199-, 2015

17 Kabeya Y, "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing" 19 : 5720-5728, 2000

18 Nishikawa T, "Inhibition of autophagy potentiates sulforaphane-induced apoptosis in human colon cancer cells" 17 : 592-602, 2010

19 Sivaprasad U, "Inhibition of ERK attenuates autophagy and potentiates tumour necrosis factor-α-induced cell death in MCF-7 cells" 12 : 1265-1271, 2008

20 Lee JW, "Induction of human leukemia U937 cell apoptosis by an ethanol extract of Dendropanax morbifera Lev. through the caspase-dependent pathway" 30 : 1231-1238, 2013

21 Mizushima N, "How to interpret LC3 immunoblotting" 3 : 542-545, 2007

22 Corcelle E, "Hofman P, Poujeol P & Mograbi B, Control of the autophagy maturation step by the MAPK ERK and p38 : lessons from environmental carcinogens" 3 : 57-59, 2007

23 Shim HJ, "Extracts from Dendropanax morbifera leaves have modulatory effects on neuroinflammation in microglia" 44 : 119-132, 2016

24 Cagnol S, "ERK and cell death : Mechanisms of ERK-induced cell death apoptosis, autophagy and senescence" 277 : 2-21, 2010

25 Lee JW, "Dendropanoxide induces autophagy through ERK1/2 activation in MG-63 human osteosarcoma cells and autophagy inhibition enhances dendropanoxide-induced apoptosis" 8 : e83611-, 2013

26 Hyo Young Jung ; 권현정 ; 한규리 ; Daeyoung Yoon ; Woosuk Kim ; Jong Whi Kim ; 김용재 ; Yeo Sung Yoon ; Dae Won Kim ; In Koo Hwang, "Dendropanax morbifera Léveille extract ameliorates cesium-induced inflammation in the kidney and decreases antioxidant enzyme levels in the hippocampus" 대한독성 유전단백체 학회 14 (14): 193-199, 2018

27 Kim W, "Dendropanax morbifera Leveille extract facilitates cadmium excretion and prevents oxidative damage in the hippocampus by increasing antioxidant levels in cadmium-exposed rats" 14 : e428-, 2014

28 Aredia F, "Autophagy and cancer" 1 : 520-534, 2012

29 Jiang F, "Artesunate induces apoptosis and autophagy in HCT116 colon cancer cells, and autophagy inhibition enhances the artesunate-induced apoptosis" 42 : 1295-1304, 2018

30 Zeng Y, "Aristolochic acid I induced autophagy extenuates cell apoptosis via ERK 1/2 pathway in renal tubular epithelial cells" 7 : e30312-, 2012

31 Elmore S, "Apoptosis : a review of programmed cell death" 35 : 495-516, 2007

32 Lee Y, "Apigenininduced apoptosis is enhanced by inhibition of autophagy formation in HCT116 human colon cancer cells" 44 : 1599-1606, 2014

33 Choi JH, "Antithrombotic effect of rutin isolated from Dendropanax morbifera Leveille" 120 : 181-186, 2015

34 Kim W, "Antioxidant effects of Dendropanax morbifera Leveille extract in the hippocampus of mercury-exposed rats" 15 : e247-, 2015

35 Choo GS, "Antiinflammatory effects of Dendropanax morbifera in lipopolysaccharidestimulated RAW264. 7 macrophages and in an animal model of atopic dermatitis" 19 : 2087-2096, 2019

36 Moon HI, "Antidiabetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats" 30 : 870-875, 2011

37 Chung IM, "Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille" 25 : 784-786, 2011

38 Hyun TK, "Anti-inflammatory activity and phenolic composition of Dendropanax morbifera leaf extracts" 74 : 263-270, 2015