Domain of Unknown Function 143 is required for the functioning of PEP-associated protein DG238 in the chloroplast

다국어 초록 (Multilingual Abstract)

Plastid-encoded plastid RNA polymerase (PEP) isessential for chloroplast development and plastid geneexpression in Arabidopsis thaliana. However, PEP is a largecomplex, and many proteins in this complex remain to beidentified. We previously reported that Delayed Greening238 (DG238) interacts with PEP subunit protein FLN1 andmay function as a PEP-associated protein and participate inearly chloroplast development and PEP-dependent plastidgene expression. DG238 contains Domain of UnknownFunction 143 (DUF143), whose function is currently unknown.
Here, we found that a deficiency of the DUF143 domain inDG238 affected its localization, which resulted in abnormalinteractions with PEP-associated proteins in the chloroplast.
Furthermore, DG238 lacking the DUF143 domain or DG238with only this domain failed to function. Interestingly, thelack of conserved amino acids 193–217 of the DUF143domain in DG238 also affected its function. In addition toFLN1, DG238 also interacts with other PEP-associatedproteins, including FSD2, FSD3, MRL7-L, and MRL7, toregulate plastid gene expression. These results suggest thatthe DUF143 domain is necessary for the functioning of thePEP-associated protein DG238 in chloroplasts.

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