연구논문 : 인공피부모델 KeraSkin(TM) 피부자극성 시험 대체시험법의 실험실간 검증 = Original Articles : Interlaboratory Validation of KeraSkin(TM) for Replacement of Skin Irritation Test Using Animal Model

다국어 초록 (Multilingual Abstract)

Reconstructed skin provides advantages to single cell culture testing and leads to promising results as shown by different validation studies. In this study we investigated the interlaboratory validation for the efficacy of “Keraskin(TM)” model. We conducted endpoint analysis including cell viability (MTT reduction) and IL-1α release. Eighteen item, which were ECVAM test meterial, were selected for this validation study. Results of MTT assay were not different between EPI-200 and Keraskin(TM), but IL-1α levels of some test items different between EPI-200 and Keraskin(TM). We concluded that this Keraskin(TM) model is useful for alternative skin irritation test, although other tests should be conducted for many other chemicals in international validation study.