당귀 추출물 정맥 주사가 Middle Cerebral Artery Occlusion 모델 흰쥐에서 Gliosis 억제에 미치는 영향 = The Effect of Intravenous Injection of the Water Extract of Angelica gigas Nakai on Gliosis in the Middle Cerebral Artery Occlusion Rats

다국어 초록 (Multilingual Abstract)

Objectives : Gliosis becomes physical and mechanical barrier to axonal regeneration. Reactive gliosis induced by middle cerebral artery occlusion is involved with up-regulation of CD81 and GFAP (Glial fibrillary acidic protein). The current study is to examine the effect of the Angelica gigas Nakai(intravenous injection. 100 mg/kg twice in a day) on CD81 and GFAP of the rat in the brain after middle cerebral artery occlusion. Methods : Cerebral infarction was induced by middle cerebral artery occlusion. And after intravenous injection of water extract of Angelica gigas Nakai, the size of cerebral infarction was measured. Examination of optical microscope were also used to detect the expression of CD81 and GFAP in the brain of the rat. Results : The following results were obtained : We found that size of cerebral infarcion induced by MCAO (Middle Cerebral Artery Occlusion) in rats were decreased after intravenous injection of Angelica gigas Nakai. We injected the extract of Angelica gigas Nakai to the MCAO in rats, and the optical microscope study showed that Angelica gigas Nakai had effect on protecting the cells of hippocampus. We found that GFAP, CD81 and ERK of the brain in rats with cerebral infarction after MCAO were meaningfully decreased after intravenous injecting Angelica gigas Nakai. We found that c-Fos expression of the brain in rats with cerebral infarction after MCAO were significantly increased after intravenous injecting Angelica gigas Nakai. Conclusions : These results indicate that Angelica gigas Nakai could suppress the reactive gliosis, which disturbs the astrocyte regeneration in the brain of the rat with cerebral infarction after MCAO by controlling the expression of CD81 and GFAP. And the effect may be modulated by the up-regulation of c-Fos and ERK.

참고문헌 (Reference)

1 한상균, "당귀약침의 혈해 자입이 Intraluminal Filament 삽입술에 의해 유발된 백서의 허혈성 뇌손상에 미치는 영향" 대한침구의학회 21 (21): 1-20, 2004

2 이승희, "당귀가 저산소로 손상된 성상세포의 gliosis 억제에 미치는 영향" 대한한의학회 29 (29): 167-178, 2008

3 Zhang SX, "X-irradiation reduces lesion scarring at the contusion site of adult rat spinal cord" 20 (20): 519-530, 2005

4 Anders JJ, "Transplanted glial scar impedes olfactory bulb reinnervation" 142 (142): 144-150, 1996

5 Puchala E, "The possibility of structural and functional restitution after spinal cord injury. A review" 55 (55): 1-42, 1977

6 Fawcett JW, "The glial scar and CNS repair" 49 (49): 377-391, 1999

7 Yang JW, "The effects of Chinese herb Angelica in focal cerebral ischemia injury in the rat" 32 (32): 209-215, 2005

8 Sung IH, "The effect of Aquaacupuncture of Radix Corydalis and Radix Angelica in endotoxin induced thrombosis in mice" 11 (11): 392-404, 1994

9 Statistics Korea, "The cause of death statistics 2009" 6-12, 2009

10 Sarthy V, "The Retinal muller cellstructure and Function" Kluwer Academic /Plenum Press 2001

11 Boucheix C, "Tetraspanins" 58 (58): 1189-1205, 2001

12 Song BK, "Temporal regulation of CD81 following retinal injury in the rat" 338 (338): 29-32, 2003

13 Oren, R, "TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins" 10 (10): 4007-4015, 1990

14 Bethea JR, "Systemically administered interleukin-10 reduces tumor necrosis factor-alpha production and significantly improves functional recovery following traumatic spinal cord injury in rats" 16 (16): 851-863, 1999

15 Hemler ME, "Specific tetraspanin functions" 155 (155): 1103-1108, 2001

16 Yauch RL, "Specific interactions among transmembrane 4 superfamily(TM4SF) proteins and phosphoinositide 4-kinase" 351 (351): 629-637, 2000

17 Aghazadeh-Habashi A, "Single Dose Pharmacokinetics and Bioavailability of Glucosamine in the Rat" 5 (5): 181-184, 2002

18 Son SY, "Shennong Ben Cao Jing" Wungwang Publishing Co 24-, 1971

19 Moon LD, "Robust regeneration of CNS axons through a track depleted of CNS glia" 161 (161): 49-66, 2000

20 Longa EZ, "Reversible middle cerebral artery occlusion without craniectomy in rats" 20 (20): 84-91, 1989

21 McKeon RJ, "Reduction of neurite outgrowth in a model of glial scarring following CNS injury is correlated with the expression of inhibitory molecules on reactive astrocytes" 11 (11): 3398-3411, 1991

22 Onodera H, "Proto-oncogene c-fos is transiently induced in the rat cerebral cortex after forebrain ischemia" 98 (98): 101-104, 1998

23 Kim GS, "Practical Clinical Guide for Western and Oriental Medicine" Jung Dam Books 22-30, 201

24 Wu YC, "Pharmacological effectgs of Radix Angelica Sinensis (Danggui) on cerebral infarction" 6 (6): 32-, 2011

25 The Korean Society of Pathologists, "Pathology" Komoonsa 1261-1262, 1990

26 Gimenez Y, "Oxysterol(7beta-hydroxycholesteryl-3-oleate) promotes serotonergic reinnervation in the lesioned rat spinal cord by reducing glial reaction" 41 (41): 79-95, 1995

27 Jung JU, "Neuroprotective effect of Angelica gigas on focal cerebral infarction in the mouse" Gyungsan University Graduate School 2000

28 Asher RA, "Neurocan is upregulated in injured brain and in cytokine-treated astrocytes" 20 (20): 2427-2438, 2000

29 Eddelstone M, "Molecular profiles of reactive astrocytes-implication for their role neurologic disease" 54 (54): 1536-, 1993

30 Masuhara K, "Involvement of extracellular signal-regulated protein kinase in gliosis induced during recovery from metabolic inhibition" 267 (267): 892-896, 2000

31 Dijkstra S, "Intraspinal administration of an antibody against CD81 enhances functional recovery and tissue sparing after experimental spinal cord injury" 202 (202): 57-66, 2006

32 Guth L, "Ineffectiveness of enzyme therapy on regeneration in the transected spinal cord of the rat" 52 (52): 73-86, 1980

33 Geisert EE Jr, "Increased brain size and glial cell number in CD81-null mice" 453 (453): 22-32, 2002

34 Bradley RR, "Hemoatopoietic effect of Radix angelicase sinensis in a hemodialysis patient" 34 (34): 349-354, 1999

35 Eng LF, "Glial fibrillary acidic protein: GFAP-thirty-one-years (1969-2000)" 25 (25): 1439-1451, 2000

36 Menet V, "GFAP null astrocytes are a favorable substrate for neuronal survival and neurite growth" 31 (31): 267-272, 2000

37 Kamii H, "Expression of c-fos mRNA after a mild focal cerebral ischemia in SOD-1 transgenic mice" 662 (662): 240-244, 1994

38 Liao JF, "Evaluation With receptor binding assay on the water extracts of ten-active herbal drugs" 19 (19): 151-158, 1995

39 Chen F, "Effects of acupointinjection of compound Angelica-root Injectio on cerebral Bcl-2 and Bax immunoactivity and hemorheology in rats with cerebral ischemiareperfusion injury" 36 (36): 85-89, 2011

40 Oh MC, "Effects of Astragali Radix and Angelicae gigantis Radix on Immune Response in Mice" 9 (9): 343-354, 1986

41 Liao WJ, "Effects of Angelica sinensis injection on the neuronal metabolites and blood flow speed within reperfusion following the ischemic cerebral injury in rats" 19 (19): 209-212, 2003

42 Berry M, "Deposition of scar tissue in the central nervous system" 32 : 31-35, 1983

43 Fidler PS, "Comparing astrocytic cells lines that are inhibitory or permissive for axon growth: the major axon-inhibitory proteoglycan in NG2" 19 (19): 8778-8788, 1999

44 Herbology Professors Association, "Clinical Herbology" Younglimsa 578-580, 1995

45 Lee SI, "Clincal Application of Herbal Medicine" Traditional Medicine Research Center 357-358, 1993

46 Xu SN, "Chinese Traditional Medical Books Translation: Shennong Ben Cao Jing" Hebei Science Technology Publising Co 68-, 1997

47 Kelic S, "CD81 regulates neuron-induced astrocyte cellcycle exit" 17 (17): 551-560, 2001

48 Levy S, "CD81 (TAPA-1): A molecule involved insignal transduction and cell adhesion in the immune system" 16 : 89-109, 1998

49 Choi MS, "Bioavailabilities of Omeprazole administered to rats through various routes" 18 (18): 141-145, 1995

50 Zou S, "Ben Jing Shu Zheng" Daesung Medical 240-245, 2001

51 Li SZ, "Ben Cao Gang Mu" People’s Medical Publishing House 833-837, 1982

52 Bahr M, "Astrocytes from adult rat optic nerves are nonpermissive for regenerating retinal ganglion cell axons" 131 (131): 211-220, 1995

53 Geisert EE Jr, "Astrocyte growth, reactivity, and the target of the antiproliferative antibody, TAPA" 16 (16): 5478-5487, 1996

54 Hu H, "Anti-inflammatory effect of radix Angelicase sinensis" 16 (16): 684-686, 1999

55 Schick MR, "Anti-TAPA-1 antibodies induce protein tyrosine phosphorylation that is prevented by increasing intracellular thiol levels" 151 (151): 1918-1925, 1993

56 전연이, "An Experimental Study of Effect on Brain Damage and Neuroprotective Effect of Angelicae Gigantis Radix extract against Cerebral Ischemia in Rats" 대한본초학회 18 (18): 4-35, 2003

57 Kwon OK, "Alteration of recovery of motor function and glial fibrillary acidic protein (GFAP) after spinal cord injury (SCI) in the rats" Gradutae School of Konkuk University 2003

58 Herrera DG, "Activation of cfos in the brain" 50 (50): 83-107, 1997

59 김강민, "Absorption, Distribution, Metabolism, and Excretion of Decursin and Decursinol Angelate from Angelica gigas Nakai" 한국미생물·생명공학회 19 (19): 1569-1572, 2009

60 Pekny M, "Abnormal reation to central nervous system injury to central nervous system injury in mice lacking glial fibrilary acidic protein and vimentin" 145 : 503-514, 1999

61 Berditchevski F, "A novel link between integrins, transmembrane-4 superfamily proteins (CD63 and CD81), and phosphatidylinositol 4-kinase" 272 (272): 2595-2598, 1997

62 Hann CK, "A Study on the Decursin content of Dang-Gwi and it’s Effect on the Hematopoiesis in Anemic Rabbit" 6 (6): 153-167, 1983