Mechanical Stretch Promotes Invasion of Lung Cancer Cells via Activation of Tumor Necrosis Factor-alpha

다국어 초록 (Multilingual Abstract)

Most of the gas exchange in the human body is carried out by the lungs, and the physiological activities of the lungs are uninterrupted. Due to the deterioration of the external environment, pulmonary cell lesions are common clinical lung diseases. Mechanical cyclic stretching is one kind of bionic technology to observe lung cancer cells. The A549 cell line is the human lung adenocarcinoma cell line derived from a primary lung tumor. This study investigated the effects of mechanical cyclic stretching on A549 cell activity and gene expression profile. Whereas mechanical cyclic stretching had no significant difference in colony formation and cell migration of A549 cells, the cell invasion increased significantly in A549 cells after stretching. In addition, the microarray data showed that mechanical cyclic stretching altered gene expression, induced inflammation of cells, and activation of Wnt/β- catenin and tumor necrosis factor pathways. More importantly, mechanical cyclic stretching activated the expression of tumor necrosis factor-alpha (TNF-α) protein. Therefore, the increase of cell invasion induced by mechanical cyclic stretching might be associated with the activation of TNF-α in human lung adenocarcinoma cells.

참고문헌 (Reference)

1 Catalano, V., "Tumor and its microenvironment: a synergistic interplay" 23 : 522-532, 2013

2 Hussain, S., "The roles of stroma-derived chemokine in different stages of cancer metastases" 11 : 598532-, 2020

3 Cheng, J., "The mechanical stress-activated serum-, glucocorticoid-regulated kinase 1 contributes to neointima formation in vein grafts" 107 : 1265-1274, 2010

4 Paul, N. E., "The effect of mechanical stress on the proliferation, adipogenic differentiation and gene expression of human adipose-derived stem cells" 12 : 276-284, 2018

5 Ho, M. Y., "TNF-α induces epithelial-mesenchymal transition of renal cell carcinoma cells via a GSK3β-dependent mechanism" 10 : 1109-1119, 2012

6 Gonzalez, D. M., "Signaling mechanisms of the epithelial-mesenchymal transition" 7 : re8-, 2014

7 Bumgarner, R., "Overview of DNA microarrays: types, applications, and their future" 2013

8 Weber, B., "Microarray-based gene expression profiling suggests adaptation of lung epithelial cells subjected to chronic cyclic strain" 33 : 1452-1466, 2014

9 Zanotelli, M. R., "Mechanoresponsive metabolism in cancer cell migration and metastasis" 33 : 1307-1321, 2021

10 Mierke, C. T., "Mechanical cues affect migration and invasion of cells from three different directions" 8 : 583226-, 2020

11 Hsia, C. C., "Lung structure and the intrinsic challenges of gas exchange" 6 : 827-895, 2016

12 Ghosh, S., "Loss of the mechanotransducer zyxin promotes a synthetic phenotype of vascular smooth muscle cells" 4 : e001712-, 2015

13 Li, Y., "Effects of direct current electric fields on lung cancer cell electrotaxis in a PMMA-based microfluidic device" 409 : 2163-2178, 2017

14 dos Santos, C. C., "DNA microarray analysis of gene expression in alveolar epithelial cells in response to TNFalpha, LPS, and cyclic stretch" 19 : 331-342, 2004

15 Zhang, B., "Cyclic mechanical stretching promotes migration but inhibits invasion of rat bone marrow stromal cells" 14 : 155-164, 2015

16 Desai, L. P., "Cyclic mechanical stretch decreases cell migration by inhibiting phosphatidylinositol 3-kinase- and focal adhesion kinase-mediated JNK1 activation" 285 : 4511-4519, 2010

17 Martino, F., "Cellular mechanotransduction: from tension to function" 9 : 824-, 2018

18 Zeltz, C., "Cancer-associated fibroblasts in desmoplastic tumors: emerging role of integrins" 62 : 166-181, 2020

19 Park, S. A., "Biological responses of ligament fibroblasts and gene expression profiling on micropatterned silicone substrates subjected to mechanical stimuli" 102 : 402-412, 2006

20 Balachandran, K., "Aortic valve cyclic stretch causes increased remodeling activity and enhanced serotonin receptor responsiveness" 92 : 147-153, 2011

21 Gong, F., "An overview of the role of mechanical stretching in the progression of lung cancer" 9 : 781828-, 2021