한국 재래종 박에서 관찰되는 바이러스 병징과 그 病原 바이러스

다국어 초록 (Multilingual Abstract)

Viral infections of twenty six accessions of Korean native bottle gourd (Lagenaria siceraria) plants were investigated. Cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic virus (ZYMV), and cucumber green mottle mosaic virus (CGMMV) were identified in the native bottle gourd plants. All examined plants were grouped into four different types of viral symptoms on the leaves. ‘Buan’ showed no visible symptom. Eighteen gourdes including ‘Andong’ showed typical yellow mosaic spots on the leaves. ‘Ulreung 1’ showed progressive wilting and severe leaf distortion. Twelve gourdes including ‘Habcheon’ showed yellow mosaic and bleaching on the leaves. Four native plant genotypes, ‘Buan’, ‘Andong’, ‘Ulreung 1’, and ‘Habcheon’, were selected to identify and to associate viruses with the typical symptoms. Antiserum against to CMV reacted positively to proteins isolated from all the selected plants. Antiserum against to CGMMV reacted positively to proteins isolated from ‘Andong’, and ‘Ulreung 1’. Antiserum against to ZYMV reacted positively to proteins isolated leaves from ‘Ulreung 1’, and ‘Habcheon’. Leaf cells of ‘Ulreung 1’ and ‘Habcheon’ showed typical potyvirus inclusions of pinwheels, scrolls and cylindrical inclusions, indicating that ZYMV was common in gourds. Mixed infection of CMV, CGMMV, and ZYMV caused extremely severe symptoms such as wilting and leaf distortion of ‘Ulreung 1’. Although ‘Buan’ showed no symptom, antiserum against CMV reacted positively on the immunblot analysis. This result suggested that ‘Buan’ might be a tolerant genotype against to plant viruses.

참고문헌 (Reference)

1 "Zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat protein gene with that of kyuri green mottle mosaic virus" 145 : 2325-2333, 2000

2 "Ultrastructural aspects of the mixed infections of watermelon mosaic potyvirus isolated from pumpkin and cucumber green mottle mosaic tobamovirus from watermelon" 16 : 216-221, 2000

3 "Ultrastructural aspects of the mixed infections of watermelon mosaic potyvirus and cucumber green mottle mosaic tobamovirus isolated from watermelon" 16 : 211-215, 2000

4 "The morphological characteristics and growth of the Korea native bottle gourd (Lagenaria siceraria Standl.) plants" 40 : 306-312, 1999(b)

5 "Selection of salt-tolerant bottle gourd (Lagenaria siceraria) rootstock for watermelon graft" 44 : 588-594, 2003

6 "Seed treatment for cucumber green mottle mosaic virus (CGMMV) in gourd (Lagenaria siceraria) seeds and its detection" 44 : 1-6, 2000

7 "RT-PCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primer" 83 : 67-73, 1999

8 "Plant virus cDNA chip hybridization for detection and differentiation of four cucurbit-infecting tobamoviruses" 110 : 19-24, 2003

9 "Occurrence of two tobamovirus diseases on cucurbits and control measures in Korea" 17 : 243-248, 2001

10 "Occurrence of cucumber green mottle mosaic virus disease of watermelon in Korea" 250-255, 1990

11 "Morphological characteristics and germination of the Korea native bottle gourd (Lagenaria siceraria Standl) seeds" 40 : 317-321, 1999(a)

12 "Isolation and identification of watermelon mosaic virus 1 and 2" 55 : 895-900, 1965

13 "Detection of the isolate of wetermelon mosaic virus and seed transmission by pollen" 8 : 131-137, 1992

14 "Cucurbit viruses: The classics and the emerging" ASHS Press 126-142, 1998

15 "Characterization of a potyvirus causing zucchini yellow mosaic virus" dgosti : 667-71 672, 1981

16 "Biological and molecular characterization of a new cucurbit-infecting Tobamovirus" 91 : 565-571, 2001