납 및 청국장 첨가식이가 흰쥐의 생육및 체내 납 분포에 미치는 영향 = Effect of Lead Chungkujang Diet on Growth and Lead Distribution in body Rat

국문 초록 (Abstract)

청국장 첨가 식이가 흰쥐의 납 독성에 미치는 영향을 규명하고자 Sprague-Dawley 계(수컷,□)흰쥐의 60마리를 대조군과 납첨가군 및 4개의 청국장 첨가군(0.25, 0.50. 1.00, 1.50%)으로 나누고, 각 실험군당 10마리로 하여 8주간 방사하였다. 그때 Pb는 100ppm의 물로 섭취케 하였으며 사육기간 별로 흰쥐의 증체량, 식이 효율과 체내 각 조직 중의 Pb축적량을 분ㅅ jr하여 청국장의 Pb축적 완화 효과를 조사하였다.
1. 흰쥐의 증체량은 청국장 첨가량이 증가할수록 약간 많았는데 그중 T_(3)(1.00%), T_(4)(1.50%)군은 대조군(Tc)보다 각각 6.22, 3.86%가 높았다(p<0.05)
2. 식이 섭취량은 대조군에 비하여 T_(2)(0.50%), T_(3)(1.00%), T_(4)(1.50%)군이 각각 0.47, 6.66, 4.27% 증가되었다.
3. 청국장을 첨가한 실험군의 평균 물 섭취량은 대조군(Tc)과 거의 같았다.
4. 각 실험군간의 식이 효율은 T_(3), T_(4)군이 Tc군보다 극소한 증가를 보였다. 각 장기들의 무게는 실험구간에 일정한 경향을 보이지 않았으나 간 및 신장의 경우 대조군(Tc)에 비하여 T3, T4군은 5.95, 9.13%가, 신장에서 각각 11.49%, 18.00%가 높았다.
5. 흰쥐의 조직 중 축적된 납의 함량은 신장>뇌>시장>간>폐>근육>고환>비장>혈액의 순으로 높았다. 체모중 납 사육량은 사육 4주째가 납 축적량이 가장 많았고 그 이후에는 감소되었다. 청국장 첨가군은 대조군에 비하여 낮았고, 특히 청국장 1.00%이상의 첨가군이 비교적 낮은 함량을 보여, 납의 축적을 억제하는 효과가 있는 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

This study was to investigate the effect of the addition of chungkukjang diet on lead toxicity of male rat (Sprague-Dawley family). There were divided into 6 different groups as follows : Control ; added lead group with 100ppm water; added chungkukjang of 0.25%, 0.50%, 1.00%, 1.50% respectively, and 10 white rats of each group were grazed for 8 weeks. The effect of chungkukjang on moderating lead accumulation was investigated by analysis of an increase in weight, diet efficiency, lead accumulation on each organ inside the body of rat.
1. The increment in weight of white rat was a little higher with increasing additional amounts of chungkukjang and T_(3), and T_(4) higher of 6.22% and 3.86% respectively than that of control group (Tc) with p<0.05
2. The amount of diet-intake was increased of 0.47, 6.66, 4.27% of t_(2), t_(3), T_(4) respectively, compared with control group (Tc).
3. The average amount of water-intake of treatment groups of additional chungkukjang was almost the same as control group (Tc).
4. The efficiency of diet of T_(3) and T_(4) was shown as a smaller increment than that of Tc. although the weight of each internal organs was not shown higher tendency regularly during the experimental period, it was shown higher of 5.95% and 9.13% in case of liver and 11.49% and 18.00% in case of kidney, respectively, compared with control group(Tc). 5. The contents of lead in the organs were as the following order : kidney > brain > heart > liver > lung > muscle > testis > spleen > blood. The content of lead accumulation was the highest in the fourth week of feeding and then it was decreased. The content of lead accumulation was lower in the additional chungkukjang groups compared with control and it was especially shown the additional group of over 1.0% chungkukjang was lower relatively, Therefore, the addition of over 1.0% chungkukjang would be an effect to inhibit the accumulation of lead.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 재료 및 방법
• 1. 재료
• 2. 방법
• Ⅲ. 결과 및 고찰
• 1. 식이섭취량, 체중증가율 및 식이효율
• 2. 물 및 Pb 섭취량과 성장률
• 3. 뇌, 각 장기 조직별, 뼈 무게 및 길이 변화
• 4. 뇌, 각 장기 조직, 근육, 뼈, 체모중 납 함량
• ·참고문헌