선충류의 주사전자현미경적 관찰을 위한 마이크로웨이브 고정법 = Microwave fixation of Setaria Digitata for scanning electron microscopy

다국어 초록 (Multilingual Abstract)

Conventional processing of biological materials including nematode parasites for scanning electron microscopy includes fixation with glutaraldehyde and osmium, followed by dehydration in an ascending grade of ethanol, and finally freeze drying. This procedure takes about 8 to 12 h depending on the characteristics of samples. Microwave irradiation of 2,450 MHz enhance the action of cross-linking fixatives and can greatly accelerate various stages of tissue processing. In this study, samples of nematode parasites, Setaria digitata, were fixed by a combination of conventional chemical fixation and the microwave irradiation during the process. The microwave irradiation was also incorporated in the serial dehydration process with ethanol. The complete procedure from the initial fixation to the completion of dehydration with ethanol was reduced to 1 h with good preservation of the ultrastructural details of the specimens.

참고문헌 (Reference)

1 신길상, "적외선 온도감응기를 장착한 마이크로파 오븐의 생체물질 고정효과" 144-145, koreanjzool1994

2 Carranza GA, "thin sectioning of tick tissues and antigen preservation in mouse spleen" 1067-1070, jmedentomol1990

3 Innes JR, "focal encephalomyelomalacia of animals caused by nematodes ; a disease which may occur in man" Cerebrospinal nematodiasis AMA Arch Neurol Psychiatry 1953 325-349,

4 Argall K, "Use of microwave fixation in the preparation of cell cultures for observation with the scanning electron microscope" 347-350, jelectronmicrosctech1990

5 Koga D, "SEM observation of the insect prepared by microwave irradiation" 52 : 477-484, 2003

6 Login GR, "Rapid primary microwave-aldehyde and microwave-osmium fixation: Improved detection of rat parotid acinar cell secretory granule α-amylase using a post-embedding immunogold ultrastructural morphometric analysis" 43 : 515-523, 1995

7 Fox NE, "Quick bacterial microwave fixation technique for scanning electron microscopy" 46 : 338-339, 1999

8 Horobin RW, "Problems and artifacts of microwave accelerated procedures in neurohistotechnology and resolutions" 15 : 101-106, 1998

9 Hernández F, "Microwave processing for scanning electron microscopy" 38 : 109-111, 2000

10 Giberson RT, "Microwave fixation: understanding the variables to achieve rapid reproducible results" 32 : 246-254, 1995

11 "Microwave fixation versus formalin fixation of surgical and autopsy tissue" 435-437,

12 Smid HM, "Microwave fixation of water-cooled insect tissues for immunohistochemistry" 313-320,

13 Hasegawa H, "Microwave fixation and localization of calcium in synaptic vesicles" (toya m) : 125-136, jneuroscimethods1994

14 Login GR, "Methods of microwave fixation for microscopy A review of research and clinical applications" 1-127, 1970-1992

15 Epperlein HH, "Improved preservation of subepidermal extracellular matrix in axolotl embryos using electron microscopical techniques based on cryoimmobilization" 118 : 43-61, 1997

16 Quissel DO, "Immunocytochemical detection of amylase in microwave fixed parotid acinar cells"

17 Mayers CP, "Histological fixation by microwave heating" 273-275, jclinpathol1970

18 Ibsch M, "Electronmicroscopic investigations on the role of vesiclelike bodies in inner maculae for fish otolith growth" 25 : 2031-2034, 2000

19 Kääb MJ, "Chondrocytes in articular cartilage preserved by rapid microwave enhanced fixation" 17 : 557-596, 1995

20 Dawes CJ, "Biological Techniques for Transmission and Scanning Electron Microscopy" 241-252, 1984

21 Umeda A, "Bacterial surfaces as revealed by the high resolution scanning electron microscope" 297-299,

22 Rhee JK, "Application of scanning electron microscopy in assessing the prevalence of some Setaria species in Korean cattle" 1-6, koreanjparasitol1994

23 Arch Histol Cytol 1988, "A new drying method of biological specimens for scanning electron microscopy" i (i): 53-59,

24 Hopwood D, "A comparison of microwaves and heat alone in the preparation of tissue for electron microscopy" 358-364,