배양된 사람 다형핵 백혈구에서 apoptosis의 연구 = Study of apoptosis in cultured human polymorphonuclear leukocytes

다국어 초록 (Multilingual Abstract)

Human polymorphonuclear leukocytes(PMN) are the primary effector cells in acute inflammation and have been implicated in the pathogenesis of a large variety of diseases. These cells have a well-established regulation and role in inflammatory response but morphologic change of human cultured PMN leukocytes is relatively uncertain. Thus, in this study, PMN leukocytes are isolated from healthy donor and cultured with times, we exammed morphologic changes of PMN leukocytes by light microscope and electron microscope and investigated DNA fragmentation and superoxide anion generation.
Survival of PMN leukocytes decreased with times. It was shown here that with time increasing number of PMN leukocytes underwent characteristic morphological changes, nuclear condensation, cytoplasmic vacuolation, nucleolar prominence and a chromatin fragmentation pattern indicative of programmed cell death or apoptosis. DNA fragmentation in cultured PMN leukocytes with time-related increased in low molecular weight chromatin. Superoxide anion generation was little decreased but decreasing rate is not more than apoptotic rate.
These results suggest that we deduce relationship between apoptotic morphologic changes and some function on removal mechanism of PMN leukocytes in inflammatory site.