치자 엑스의 가수분해에 의한 피부투과 및 항염효과 = Skin Permeation and Anti-inflammatory Effects of Hydrolyzed Products of Gardeniae Fructus Extracts

다국어 초록 (Multilingual Abstract)

Geniposide and its related iridoid compounds have been used in traditional herbal medicine for the treatment of Jaundice hepatic diseases and various inflammatorys. For the purpose to increase trandsdermal absorption, the hydrolyzed products of Gardeniae Furcuts were identified and assayed of active ingredients and investigated trandsdermal absorption and anti-inflammatory effects. Geniposide was hydrolyzed to genipin by β-glucosidase and it was suggested that genipin was more suitableform than geniposide for transdermal absorption by its lipophilic property. Using Franz type diffusion cell and the skin of hairless mouse, the permeation rate of hydrolyzed products and their emulsion preparation were determined. Genipin have more increased absorption ratio through the skin hairless mouse than geniposide. Also, the emulsion of hydrolyzed products of extracts showed higher permeability than that of nonhydrolyzed preparations. After 9 hours 280.85 ㎍/㎠ of genipin was absorbed and 193.52 ㎍/㎠ in case of geniposide. The Js of geniposide and genipin were 26.27±4.11 ㎍/㎠/hr and 40.35±5.04 ㎍/㎠/hr respectively. After carrageenan injection, the swelling was increased rapidly to 24 hr and maintained as a plateau. But emulsion group were reached about 2.5 mL and the swelling decreased successively from 24 hr to 72 hr. The anti-inflammation effects of extracts and hydrolyzed products emulsion were increased with significant difference with control group after 24 hr, 48 hr and 72 hr. In carrageenan induced edema, inhibition of swelling was increased in case of hydrolyzed product emulsion compare with nonhydrolyzed group at 24 hr, 48 hr and 72 hr after swelling. In histological study, the anti-inflammatory effects of hydrolyzed products were remarkable at 48 hr and 72 hr compare with nonhydrolyzed. Hydrolyzed products of Gardeniae Frucuts extract containing genipin would be a suitable preparation to increase the transdermal absorption and anti-inflammatory effects.

참고문헌 (Reference)

1 "Y.B. Chung and Y.S. Kang" 114-, 1993

2 "Transdermal delivery of triamcinolone acetonide gel by ultrasound" 2002

3 "Sung, In vivo evaluation of celluar and acellular bovine pericadia fixed with a naturally occurring crosslinking agent(genipin)" 12 (12): 851-852, 2001

4 "Studies on Scutellariae Radix Effects on ethanol-induced hyperlipemia and lipolysis in isolated fat cells." 219-222, 1982

5 "Physical stability of the blue pigments formed from geniposide of gardenia fruits: effects of pH, temperature and ligh" 49 (49): 430-432, 2001

6 "Phase solubility technics" 117-212, 1965

7 "Pharmacokinetic and pharmacological evaluation of topical microemulsion prepara- tion containing piroxicam" 7 : 54-58, 1999

8 "Interaction of anti-inflammatory drugs in carrageenan-induced foot edema of the rat" 172 : 423-425, 1974

9 "In vitro surface characterization of a biological patch fixed with naturally occurring crosslinking agent" 21 (21): 1353-1362, 2000

10 "In vitro evaluation of a chitosan membrane cross-linked with genipin" 12 (12): 835-850, 2001

11 "Improvement of hydrolysis and bioavailability of coprecipitated products of coptidis Rhizoma and scutellariae Radix by b-glucuronidase" 33 (33): 91-97, 2003

12 "Identification and determination of geniposide and geniposidic acid from herbs by HPLC/photodiode-array detection" garde (garde): 2199-2205, 1994

13 "HPLC determination of iridoids in cape jasmine fruit" 298-299, 1993

14 "Formation of nitrogen containing metabolites from genipo- side and gardenoside by human intestinal bacteria" 57 : 536-542, 1991

15 "Enzymatic studies on the animal and intestinal bacterial methabolism of geniposide" 17 : 1573-1576, 1994

16 "Enhancing effect of pyrrolidone derivatives on transdermal penetration of phenolsulfophthalcin and indomethacin from aqueous vehicle" 38 (38): 797-799, 1990

17 "Effects of heparin immobilization on the surface characteri- stics a biological tissue fixed with a naturally occurring crosslinking agent (genipin) an in vitro study" 22 (22): 523-533, 2001

18 "Effect and mode of action of aliphatic esters on the in vitro skin permeation of nicorandil" 43 : 31-40, 1988

19 "Determination of geniposide geni- posidic acid and genipin-1-beta-gentiobioside in gardenia jasminoides by high performance liquid chromatography" garde (garde): 410-414, 1988

20 "Dermatological Formulations" new york : 49-94, 1983

21 "Antioxi- dant components of gardenia Fruits" 25 (25): 226-232, 1994