<i>Gracilimonas rosea</i> sp. nov., isolated from tropical seawater, and emended description of the genus <i>Gracilimonas</i>

다국어 초록 (Multilingual Abstract)

<P>A Gram-staining-negative, non-motile, spore-forming, rod-shaped, marine bacterial strain, CL-KR2<SUP>T</SUP>, was isolated from tropical seawater near Kosrae, an island in the Federated States of Micronesia. Analysis of the 16S rRNA gene sequence of strain CL-KR2<SUP>T</SUP> revealed a clear affiliation with the genus <I>Gracilimonas</I>. Based on phylogenetic analysis, strain CL-KR2<SUP>T</SUP> showed the closest phylogenetic relationship to <I>Gracilimonas tropica</I> CL-CB462<SUP>T</SUP>, with 16S rRNA gene sequence similarity of 96.6 %. DNA–DNA relatedness between strain CL-KR2<SUP>T</SUP> and <I>G. tropica</I> CL-CB462<SUP>T</SUP> was 6.7 % (reciprocal 9.5 %). Strain CL-KR2<SUP>T</SUP> grew in the presence of 1–20 % sea salts and the optimal salt concentration was 3.5–5 %. The temperature and pH optima for growth were 35 °C and pH 7.5. The major cellular fatty acids (≥10.0 %) of strain CL-KR2<SUP>T</SUP> were iso-C<SUB>15 : 0</SUB>, summed feature 3 (iso-C<SUB>15 : 0</SUB> 2-OH and/or C<SUB>16 : 1</SUB>ω7<I>c</I>) and iso-C<SUB>17 : 1</SUB>ω9<I>c</I> and the only isoprenoid quinone was MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, two unidentified glycolipids and two unidentified lipids. The genomic DNA G+C content of strain CL-KR2<SUP>T</SUP> was 43.2 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-KR2<SUP>T</SUP> could be distinguished from the only member of the genus <I>Gracilimonas</I> with a validly published name. Thus, strain CL-KR2<SUP>T</SUP> should be assigned to a novel species in the genus <I>Gracilimonas</I>, for which the name <I>Gracilimonas rosea</I> sp. nov. is proposed. The type strain is CL-KR2<SUP>T</SUP> ( = KCCM 90206<SUP>T</SUP> = JCM 18898<SUP>T</SUP>).</P>