백두웅 추출물의 항균활성에 관한 연구 = Study on the Antimicrobial Activities of the Extracts from Pulsatillae Radix

국문 초록 (Abstract)

목적 : 항생제 내성균들의 등장으로 새로운 항생제의 개발이 요구되고 있는 상황에서 본 연구는 천연 항균제를 개발하기 위한 일환으로 살균작용이 있다고 알려진 한약제 중의 하나인 백두옹에서 항균물질을 분리 시도하였다.
방법 : 박두옹을 hexane, dichloromethane, methanol 및 증류수로 연속적으로 추출하여 각 추출물의 항균력 보유 여부를 조사하였고 물질의 분리를 위하여 liquid column chromatography와 thin layer chromatography를 실시하였고 물질의 질량분석을 위하여 mass spectrometry를 의뢰 실시하였고 그 결과를 NIST lubrary 검색을 통하여 분리된 물질을 규명하였다.
결과 : Hezane 추출물은 Staphylococcus aureus, MRSA, Proteus vulgaris, Klebsiella pneumoniae에 대하여 항균 활성을 나타내었고, dichloromethane 추출물은 Staphylococcus aureus, MRSA, Bacillus subtilis, Proteus vulgaris, Klebsiella pneumoniae에 대하여 추출물들 중 가장 높은 항균 효과를 나타내었다. methanol 추출물은 dichloromethane 추출물보다 항균력이 낮았으며 물 추출물의 항균활성은 methanol 추출물과 거의 비슷하였다. 분리 정제된 dichloromethane 추출물은 Cadida albicans에 대해서도 항균 효과를 나타내었다.
결론 : 천연 항균제를 개발하기 위한 일환으로 백두옹을 hexane, dichloromethan,e methanol 및 증류수로 추출하였고 dichlorometnane 추출물이 그람 양성균과 MRSA에 대하여 가장 높은 항균력을 나타내었고 정제된 dichloromethane 추출물은 Candida albicans에 대해서도 항균 효과를 나타내었다. Mass spectrometry와 MIST(National Institute of Standards and Technology) library 검색 결과 분리된 물질이 9. 12-octadecadienoic acid일 가능성이 있는 것으로 보여진다.

다국어 초록 (Multilingual Abstract)

Abuse and inappropriate use of antibiotics have resulted in emergence of antibiotic-resistant microorganisms, increased nosocomial infections and urgency of developing new drugs effectively coping the resistant microoganisms. Recently infections caused by methicillin-resistant Staphylococcus aureus(MRSA) with reduced susceptibility to vancomycin have been reported in both Japan and the Unites States. There have been many reports about screening plants for antimicrobial properties. In this study, extracts from Pulsatillae Radix were examined for antimicrobial activities.
Methods : Pulsatillae Radix, dried roots of Pulsatilla koreana, was extracted with hexane, dichloromethane, methanol, distilled water, respectively and serially. Liquid column chromatography and thin layer chromatography were used to separate the materials with antimicrobial activities, and mass spectrometry was used to determine the mass.
Results : From 500g of Pulsatillae Radix, 1.2g of extract was obtained by hexane extraction, 2.5g by Dichloromethane, 73.9g by methanoi and 57.6g by water. Hexane extract revealed antimicrobial activities against Staphylococcus aureus, MRSA, Proteus vulgaris. Klebsiella pneumoniae. Dichlorometnane extract showed the highest antimicrobial activites against Staphlococcus aureus, MRSA, Bacillus subtilis, Proteus vulgaris, Klebsiella pneumoniae. Antimicrobial activity of methanoi extract were lower than dichloromethane extract. Water extract showed nearly similar activity with methanoi extract. Purified dichlomethane extracts only revealed antimicrobial activity against Candida albicans.
Conclusion : As a step to develop natural antimicrobial agents, Pulsatillae Radix was extracted with hexane, dichloromethane, methanoi and water. Dichloromethane extract showed highest antimicrobial activities against gram-postive bacteria, especially MRSAs.
Purified dichloromethane extract even also revealed antimicroobial activity aganist Candida albicans. Mass spectrophotometry and survey of NIST(National Insitute Standards and Technology) library suggested that this extract may be 9, 12-octadecadienoic acid.