자연과학편 : 운동부하가 흰쥐의 골격근내 지질대사율과 UCP-3 및 CPT-1 단백질 발현에 미치는 영향 = The Effect of Different Exercise Intensity on Lipid Metabolism, UCP-3 and CPT-1 Protein Expression Skeletal Muscle in Rats

다국어 초록 (Multilingual Abstract)

The purpose of this study was to investigate the gene expression related with lipid metabolism according to intensity and duration of treadmill exercise in rats. Sprague-Dawley rats were randomly divided into control(CON), high -intensity(H-EX) and low-intensity exercise(L-EX) groups. Rats in L-Ex group were forced to run on the treadmill at the speed of 5m/min for initial 10 min and 10m/min for next 20 min. On the other hand, rats in the H-Ex group were forced to run on the treadmill slope 0 at the speed of 16m/min for initial 10 min, 22m/min for next 20 min, and 25m/min for last 40 min.


Expression of UCP3 of the WG and RG in L-Ex and H-Ex groups was increased compared with control immediately after exercise. Expression of UCP3 of the WG and RG in the H-Ex group was higher than in the L-Ex group.


CPT-1 expression of the WG and RG in the H-Ex group was increased immediately and 6 hr after exercise compared with control. CPT-1 expression of the RG in the H-Ex group was highest 6hr after exercise. CPT-1 expression of the RG and WG in the L-Ex group was not differ among groups.


Palmitate oxidation rate of the RG and WG was increased 0, 6 and 12 hr after exercise in the H-Ex group, and 0 and 6 hr after exercise in the L-Ex group compared with control. Palmitate oxidation rate of the H-Ex group was higher than in the L-Ex group 0, 6 and 12 hr after exercise in RG, and 0 and 6hr after exercise in WG.In the summary, gene expression of UCP3 and CPT1 that related with lipid metabolism was not significantly affected during 0 to 60 min recovery time by low-intensity exercise, but effected by high-intensity exercise.


In conclusion, exercise intensity and amounts might be have very important role to regulate gene expression related with metabolism.