Relationships among bedding materials, bedding bacterial composition and lameness in dairy cows

다국어 초록 (Multilingual Abstract)

Objective: Bedding materials directly contact hooves of dairy cows and they may serve as environmental sources of lameness-associated pathogen. However, the specific composition of bacteria hidden in bedding materials is still not clear. The aim of this study was to determine the effect bedding material and its bacterial composition has on lameness of Holstein heifers. Methods: Forty-eight Holstein heifers with similar body weights were randomly assigned into three groups including sand bedding (SB), concrete floor (CF), and compost bedding (CB). Hock injuries severity and gait performance of dairy cows were scored individually once a week. Blood samples were collected at the end of the experiment and bedding material samples were collected once a week for Illumina sequencing. Results: The CF increased visible hock injuries severity and serum biomarkers of joint damage in comparison to SB and CB groups. Besides, Illumina sequencing and analysis showed that the bacterial community of CB samples had higher similarity to that of SB samples than CF samples. Bacteria in three bedding materials were dominated by gastrointestinal bacteria and organic matter-degrading bacteria, such as Actinobacteria, Firmicutes, and norank JG30-KF-cM45. Lameness-associated Spirochaetaceae and Treponeme were only detected in SB and CB samples with a very low relative abundance (0% to 0.08%). Conclusion: The bacterial communities differed among bedding materials. However, the treponemes pathogens involved in the pathogenesis of lameness may not be a part of microbiota in bedding materials of dairy cows.

참고문헌 (Reference)

1 Garnero P, "Uncoupling of type II collagen synthesis and degradation predicts progression of joint damage in patients with knee osteoarthritis" 46 : 2613-2624, 2002

2 Li J, "Substrate type and free ammonia determine bacterial community structure in full-scale mesophilic anaerobic digesters treating cattle or swine manure" 6 : 1337-, 2015

3 Cole KJ, "Short communication : environmental mastitis pathogen counts in freestalls bedded with composted and fresh recycled manure solids" 99 : 1501-1505, 2016

4 Nielsen MW, "Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples" 186 : 139-149, 2016

5 Zhao XJ, "Oxidative stress and imbalance of mineral metabolism contribute to lameness in dairy cows" 164 : 43-49, 2015

6 Lu Y, "Mucosal adherent bacterial dysbiosis in patients with colorectal adenomas" 6 : 26337-, 2016

7 Klitgaard K, "Microbiota analysis of an environmental slurry and its potential role as a reservoir of bovine digital dermatitis pathogens" 83 : e00244-17-, 2017

8 Landewé R, "Markers for type II collagen breakdown predict the effect of disease-modifying treatment on long-term radiographic progression in patients with rheumatoid arthritis" 50 : 1390-1399, 2004

9 Cook NB, "Management characteristics, lameness, and body injuries of dairy cattle housed in high-performance dairy herds in Wisconsin" 99 : 5879-5891, 2016

10 Ito K, "Lying behavior as an indicator of lameness in dairy cows" 93 : 3553-3560, 2010

11 Randall LV, "Lameness in dairy heifers; impacts of hoof lesions present around first calving on future lameness, milk yield and culling risk" 133 : 52-63, 2016

12 Evans NJ, "Host and environmental reservoirs of infection for bovine digital dermatitis treponemes" 156 : 102-109, 2012

13 Kim JY, "Genomic analysis of a pathogenic bacterium, Paeniclostridium sordellii CBA7122 containing the highest number of rRNA operons, isolated from a human stool sample" 8 : 840-, 2017

14 Norring M, "Effects of sand and straw bedding on the lying behavior, cleanliness, and hoof and hock injuries of dairy cows" 91 : 570-576, 2008

15 Flower FC, "Effect of hoof pathologies on subjective assessments of dairy cow gait" 89 : 139-146, 2006

16 Oehme B, "Effect of different flooring types on pressure distribution under the bovine claw–an ex vivo study" 14 : 259-, 2018

17 Miguel-Pacheco GG, "Effect of claw horn lesion type and severity at the time of treatment on outcome of lameness in dairy cows" 225 : 16-22, 2017

18 Klitgaard K, "Discovery of bovine digital dermatitis-associated Treponema spp. in the dairy herd environment by a targeted deep-sequencing approach" 80 : 4427-4432, 2014

19 Krull AC, "Deep sequencing analysis reveals temporal microbiota changes associated with development of bovine digital dermatitis" 82 : 3359-3373, 2014

20 Ridaura VK, "Contextual control of skin immunity and inflammation by Corynebacterium" 215 : 785-799, 2018

21 Liu J, "Comparative studies of the composition of bacterial microbiota associated with the ruminal content, ruminal epithelium and in the faeces of lactating dairy cows" 9 : 257-268, 2016

22 Ming L, "Comparative analysis of fecal microbial communities in cattle and Bactrian camels" 12 : e0173062-, 2017

23 Burgstaller J, "Claw health and prevalence of lameness in cows from compost bedded and cubicle freestall dairy barns in Austria" 216 : 81-86, 2016

24 Corona PS, "Case report : first report of a prosthetic joint infection caused by Facklamia hominis" 80 : 338-340, 2014

25 Skiöldebrand E, "Cartilage oligomeric matrix protein neoepitope in the synovial fluid of horses with acute lameness : a new biomarker for the early stages of osteoarthritis" 49 : 662-667, 2017

26 Rowbotham RF, "Association of bedding types with management practices and indicators of milk quality on larger Wisconsin dairy farms" 98 : 7865-7885, 2015

27 Zinicola M, "Altered microbiomes in bovine digital dermatitis lesions, and the gut as a pathogen reservoir" 10 : e0120504-, 2015

28 Han Z, "A novel spatiotemporally anaerobic/semi-aerobic bioreactor for domestic solid waste treatment in rural areas" 86 : 97-105, 2019

29 Maboni G, "A distinct bacterial dysbiosis associated skin inflammation in ovine footrot" 7 : 45220-, 2017

30 Bay V, "16S rRNA amplicon sequencing reveals a polymicrobial nature of complicated claw horn disruption lesions and interdigital phlegmon in dairy cattle" 8 : 15529-, 2018