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    Development of Cre-lox based multiple knockout system in Deinococcus radiodurans R1

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    https://www.riss.kr/link?id=A103562461

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    The extremophilic bacterium Deinococcus radiodurans R1 has been considered as an attractive microorganism due to its remarkable tolerance to various external stresses. Considering the nature of D. radiodurans R1, it has potential as a platform microorganism for industrial applications, including biorefinery and bioremediation process.
    However, D. radiodurans R1 is well known for its hard genetic manipulation. Thus, much effort has been made to develop efficient genetic engineering tools for making D. radiodurans R1 suitable for industrial platform microorganism.
    Although a plasmid-based single gene knockout method has been reported, development of multiple gene knockout system has not yet been reported. Here we report, for the first time, Cre-lox based rapid and efficient multiple knockout method for metabolic engineering of D. radiodurans R1. Also, deletion of dr0053 gene was successfully achieved within seven days to make biofilm overproducing strain.
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    The extremophilic bacterium Deinococcus radiodurans R1 has been considered as an attractive microorganism due to its remarkable tolerance to various external stresses. Considering the nature of D. radiodurans R1, it has potential as a platform microor...

    The extremophilic bacterium Deinococcus radiodurans R1 has been considered as an attractive microorganism due to its remarkable tolerance to various external stresses. Considering the nature of D. radiodurans R1, it has potential as a platform microorganism for industrial applications, including biorefinery and bioremediation process.
    However, D. radiodurans R1 is well known for its hard genetic manipulation. Thus, much effort has been made to develop efficient genetic engineering tools for making D. radiodurans R1 suitable for industrial platform microorganism.
    Although a plasmid-based single gene knockout method has been reported, development of multiple gene knockout system has not yet been reported. Here we report, for the first time, Cre-lox based rapid and efficient multiple knockout method for metabolic engineering of D. radiodurans R1. Also, deletion of dr0053 gene was successfully achieved within seven days to make biofilm overproducing strain.

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    참고문헌 (Reference)

    1 R. Meima, 66 : 3856-, 2000

    2 E. Gerber, 119 : 1-, 2015

    3 G. Stephanopoulos, 1 : 514-, 2012

    4 V. G. Vikramaditya, 14 : 233-, 2012

    5 K. S. Makarova, 65 : 44-, 2001

    6 S. Singh, 19 : 437-, 2008

    7 J. K. Grimsley, 60 : 613-, 1991

    8 H. Brim, 18 : 85-, 2000

    9 H. Brim, 152 : 2469-, 2006

    10 K. Abremski, 32 : 1301-, 1983

    1 R. Meima, 66 : 3856-, 2000

    2 E. Gerber, 119 : 1-, 2015

    3 G. Stephanopoulos, 1 : 514-, 2012

    4 V. G. Vikramaditya, 14 : 233-, 2012

    5 K. S. Makarova, 65 : 44-, 2001

    6 S. Singh, 19 : 437-, 2008

    7 J. K. Grimsley, 60 : 613-, 1991

    8 H. Brim, 18 : 85-, 2000

    9 H. Brim, 152 : 2469-, 2006

    10 K. Abremski, 32 : 1301-, 1983

    11 H. H. Nguyen, 73 : 240-, 2009

    12 R. Hoess, 181 : 351-, 1985

    13 H. Ohba, 363 : 133-, 2005

    14 R. R. Kumar, 51 : 403-, 2011

    15 K. Satoh, 62 : 1-, 2009

    16 D. Appukuttan, 10 : e0118275-, 2015

    17 G. A. O’Toole, 310 : 91-, 1999

    18 B. R. Boles, 4 : e1000052-, 2008

    19 B. E. Moseley, 61 : 176-, 1968

    20 J. M. Lambert, 73 : 1126-, 2007

    21 R. Dixit, 7 : 2189-, 2015

    22 M. Kolari, 27 : 343-, 2001

    23 R. Shashidhar, 56 : 195-, 2010

    24 D. Slade, 75 : 133-, 2011

    25 L. Lemee, 53 : 919-, 1997

    26 노창현, "Microbial bioremediation processes for radioactive waste" 한국화학공학회 32 (32): 1720-1726, 2015

    27 Jacintha Esther, "Fe (III) reduction strategies of dissimilatory iron reducing bacteria" 한국화학공학회 32 (32): 1-14, 2015

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    학술지 이력

    학술지 이력
    연월일 이력구분 이력상세 등재구분
    2023 평가 해외DB학술지평가 신청대상 (해외등재 학술지 평가)
    2020-01-01 등재 등재학술지 유지 (해외등재 학술지 평가) KCI등재
    2016-06-21 학술지명변경 한글명 : The Korean Journal of Chemical Engineering -> Korean Journal of Chemical Engineering
    외국어명 : The Korean Journal of Chemical Engineering -> Korean Journal of Chemical Engineering
    KCI등재
    2011-01-01 등재 등재학술지 유지 (등재유지) KCI등재
    2009-01-01 등재 등재학술지 유지 (등재유지) KCI등재
    2007-09-27 학회명변경 영문명 : The Korean Institute Of Chemical Engineers -> The Korean Institute of Chemical Engineers KCI등재
    2007-09-03 학술지명변경 한글명 : The Korean Journal of Chemical Engineeri -> The Korean Journal of Chemical Engineering
    외국어명 : The Korean Journal of Chemical Engineeri -> The Korean Journal of Chemical Engineering
    KCI등재
    2007-01-01 등재 등재학술지 유지 (등재유지) KCI등재
    2005-01-01 등재 등재학술지 유지 (등재유지) KCI등재
    2002-01-01 등재 등재학술지 선정 (등재후보2차) KCI등재
    1999-07-01 등재 등재후보학술지 선정 (신규평가) KCI등재후보
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    학술지 인용정보

    학술지 인용정보
    기준연도 WOS-KCI 통합IF(2년) KCIF(2년) KCIF(3년)
    2016 1.92 0.72 1.4
    KCIF(4년) KCIF(5년) 중심성지수(3년) 즉시성지수
    1.15 0.94 0.403 0.14
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