마우스 대장암 모델 구축 및 항암제 활성 평가를 위한 예비 연구 = Preliminary Study for Establishment of Preneoplastic Model of Colon in Mice and Evaluation of Anticancer Effects

다국어 초록 (Multilingual Abstract)

Abstract – Aberrant crypt foci (ACF) are early imorphological changes observed in rodents after administration of colon-specific carcinogen such as azoxymethane (AOM). ACF are considered to be putative preneoplastic lesions and are widely used as a surrogate biomarker to rapidly evaluate chemopreventive potential
of compounds. The size of colorectal cancer was evaluated after administration of three anticancer drugs, 1
parent drug and 2 prodrugs. The body weights of mice were measured daily and considered as a surrogate
for evaluation of general wellbeing. Colons were removed, cut along the longitudinal axis and flushed with
phosphate-buffered saline. Each colon was cut into three equal lengths and fixed flat between filter papers.
The fixed colon sections were stained with methylene blue. The number of ACF per colon, the number of
aberrant crypts observed in each focus and the location of each focus were recorded. After single administration
of AOM and multiple doses of anticancer drugs, no significant changes in the body weights of the mice was observed which was recorded for 52 days. However, an expected ACF was not observed in any treated groups. These findings suggest the induction of ACF in mice requires the promotion by dextran sulfate sodium as well as the initiation by AOM.