Comparison of the Concentrations of 8 - MOP in both Plasma and Suction Blister Fluid after Oral Ingestion = Comparison of the Concentrations of 8 - MOP in both Plasma and Suction Blister Fluid after Oral Ingestion

다국어 초록 (Multilingual Abstract)

Background: The value of plasma concentration of 8-Methoxypsoralen (8-MOP) in the supervision of photochemotherapy has been recognized. However, plasma levels of 8-MOP were not proportionate to the degree of PUVA induced erythema and couldn't alone predict the degree of PUVA induced erythemal reaction. We made a speculation that the degree of PUVA induced erythema might correlate better with skin tissue levels of 8-MOP than plasma levels. Suction blister fluid (SBF) has been known to represent tissue fluid in the skin. So we performed a study of comparison of 8-MOP concentrations in both plasma and SBF. Objective: Our purpose was to evaluate the correlation of the concentrations of 8-MOP in plasma and SBF 2 hours after oral administration of 0.6 mg/kg of 8-MOP. Methods: Twenty six patients, aged between 16 and 50 years, undergoing suction blister surgery for vitiligo treatment, participated in this open study. Single oral doses of 0.6 mg/kg of body weight of 8-MOP were taken. Blood samples (5ml) and SBF (2ml) were collected at 2 hours after the drug administration, and 8-MOP concentration in plasma and SBF were quantitated by reverse phase high-performance liquid chromatography (HPLC). Results:8-MOP concentrations in plasma and SBF ranged from 18 to 545 ng/ml and 8 to 179 ng/ml, respectively. On the analysis of linear regression, a close relation could not be observed between two SBF 1evels; measured and predicted values which were calculated from measured plasma and SBF concentrations (r2=0.583, P < 0.001). Conclusion: The correlation of plasma and SBF concentrations of 8-MOP is weak. So, SBF levels of psoralen are recommended for the study of PUVA erythemal reactions. (Ann Dermatol 11(4) 218~224,1999).