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      난소암 환자 복수내의 면역복합체로부터 새로운 암관련항원 존재 확인 = Isolation of Ovarian Tumor-associated Antigens from Ascitic Fluid of Patients with Ovarian Carcinoma

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      https://www.riss.kr/link?id=A2064824

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      Immune complexes were isolated from ascitic fluid of patients with ovarian carcinoma by using protein-A Sepharose CL-4B affinity chromatography, and antigen-antibody complexes then dissociated by ion exchange chromatography in the presence of 8M urea. Only one antigen fraction was obtained by the ion exchange chromatography, but three antigenic bands were demonstrated by SDS-PAGE. The molecular weights of the three protein bands were 70, 41, and 38 kDa, respectively. Of the three proteins the 38 kDa band has already been known, but 70 and 41 kDa bands seems to represent new ovarian tumorassociated antigens.
      This study may suggest the involvement of multiple antigens in ovarial tumor which can be included in producing tumor-directed monoclonal antibodies
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      Immune complexes were isolated from ascitic fluid of patients with ovarian carcinoma by using protein-A Sepharose CL-4B affinity chromatography, and antigen-antibody complexes then dissociated by ion exchange chromatography in the presence of 8M urea....

      Immune complexes were isolated from ascitic fluid of patients with ovarian carcinoma by using protein-A Sepharose CL-4B affinity chromatography, and antigen-antibody complexes then dissociated by ion exchange chromatography in the presence of 8M urea. Only one antigen fraction was obtained by the ion exchange chromatography, but three antigenic bands were demonstrated by SDS-PAGE. The molecular weights of the three protein bands were 70, 41, and 38 kDa, respectively. Of the three proteins the 38 kDa band has already been known, but 70 and 41 kDa bands seems to represent new ovarian tumorassociated antigens.
      This study may suggest the involvement of multiple antigens in ovarial tumor which can be included in producing tumor-directed monoclonal antibodies

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