섬오가피에 대한 항돌연변이원 시험 = Antimutagenic Study on Acanthopanax Koreanum Nakai

다국어 초록 (Multilingual Abstract)

This study was undertaken to investigate the mutagenicity and antimutagenicity of Acanthopanax koreanum Nakai. Antimutagenic study on extract of A. koreanum was studied using the test with Salmonella typhimurium TA100, TA98. And mutagenicity study was studied using the test with S. typhimurium TA100, TA98, TA1535,TA1537 and Escherichia coli WP2 uvr A. A. koreanum was negative in Ames test with S. typhimurium and E. coli with or without S-9 mixture. Test substances of 5000 μg/μl, 2500 μg/μl and 600 μg/μl of A. koreanum extracts were chosen via toxicity test. Ames test was performed on positive control group, experimental group and negative control group in the presence of the metabolic activation system and metabolic non-activation system. As a result, there was no coherent increase and reverse mutation in all concentrations. Therefore, A. koreanum does not cause reverse mutation.
In addition, A. koreanum showed strong antimutagenic activities in S. typhimurim TA100 and TA98. In conclusion,A. koreanum root may be an excellent antimutagenic agent.

참고문헌 (Reference)

1 임자훈, "탐라오갈피의 침출 중 유용성분의 변화" 한국응용생명화학회 48 (48): 166-172, 2005

2 임병선, "지리산오갈피와 섬오갈피의 생육 특성 및 자생지 식생조사" 12 : 125-132, 1999

3 이상현, "오갈피나무의 부위별 Eleutheroside B와 Eleutheroside E의 정량" 한국생약학회 36 (36): 70-74, 2005

4 박소영, "약이 되는 나무: 섬오갈피나무" 산림조합중앙회 112-114, 2005

5 고한종, "섬오갈피나무의 발아 및 유묘의 생육특성" 한국약용작물학회 11 (11): 46-52, 2003

6 이상은, "산화적 스트레스에 대한 섬오갈피 메탄올 추출물의 보호 효과" 한국생약학회 37 (37): 16-20, 2006

7 박금주, "가시오가피 추출물의 광노화에 의한 주름형성 억제 효과" 한국식품영양과학회 39 (39): 42-46, 2010

8 Maron, D., "Revised methods for the Salmonella mutagenicity test" 113 : 173-215, 1983

9 Lee, J.Y., "Mutagenicity of thermally oxidized soybean oil" 32 : 1213-1220, 2000

10 Ames, B., "Methods for detecting carcinogens and mutagens with the Salmonella/mammalianmicrosome mutagenicity test" 31 : 347-364, 1975

11 Gomes-Carneiro, M.R., "Evaluation of mutagenic and antimutagenic activities of alpha-bisabolol in the Salmonella/microsome assay" 585 : 105-112, 2005

12 Choi, Y.H., "Antimutagenic effects of the juice and boiling water extract of Houttuynia cordata Thunb" 23 : 916-921, 1994

13 Yang, E.J., "Acanthopanax koreanum fruit waste inhibits lipopolysaccharide-induced production of nitric oxide and prostaglandin E2 in RAW 264.7 macrophages" 2010 : 715-739, 2010

14 한덕룡, "Acanthopanax koreanum Nakai의 약효성분에 대한 연구" 29 : 357-361, 1985