Expression of Fanconi Anemia D2 Gene and Its Function in Hela and MCF10A Cells

다국어 초록 (Multilingual Abstract)

Fanconi Anemia (FA) is a disease with autosomal recessive inheritance and characterized by
developmental abnormalities, progressive bone marrow failure and cancer predisposition.
Especially, the phenotypes of FA cells show the extreme sensitivity towards oxygen and DNA
crosslinking agents such as diepoxybutane and mitomycin C (MMC). In the current study, I
made the retroviruses which expressed FANCD2 gene for making the stable cell lines, Hela
(cervical carcinoma) and MCF10A (breast). I could detect the expression of FANCD2 protein
in the Hela and MCF10A stable cells after the puromycin selection. When I incubated the
cells lines with crosslinking agents, MMC, the MCF10A cells which expressed the exogenous
FANCD2 was resistant to the MMC, compared with the MCF10 parent cells. But the Hela
cells which expressed the FANCD2 did not show the resistance to the MMC. Together, I
conclude that FANCD2 protein is an important factor for resistance to the crosslinking agent,
MMC in the MCF10A breast cell, but not the Hela cervical carcinoma cells.

참고문헌 (Reference)

1 "Unusual response to bifunctional alkylating agents in a case of Fanconi anaemia" 384-387, 1983

2 "The emerging genetic and molecular basis of Fanconi anaemia" 2: : 446-457, 2001

3 "The Fanconi anaemia/BRCA pathway. Nat. Rev" 3 : 23-34, 2003

4 "Pathways of DNA double-strand break repair and their impact on the prevention and formation of chromosomal aberrations" ge 104 (ge 104): 7-, 2004

5 "Mutation in Brca2 stimulates eror-prone homology- directed repair of DNA double-strand breaks occurring between repeated sequences" 20: : 4704-20 4716, 2001

6 "Molecular views of recombination proteinsand their control" 4: : 435-445, 2003

7 "Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway" 7 : 249-7 262, 2001

8 "Genetic basis of Fanconi anemia" 10: : 68-76, 2003

9 "Evidence for at least eight Fanconi anemia genes" 61 : 940-944, 1997

10 "Direct interactions of the five known Fanconi anaemia proteins suggest a common functional pathway" 10: : 423-429, 2001

11 "Direct interaction of the Fanconi anaemia protein FANCG with BRCA2/FANCD1" 12 : 2503-2510, 2003

12 "Direct interaction of FANCD2 with BRCA2 in DNA damage response pathways" 13 : 1241-1248, 2004

13 "Deficient DNA end joining activity in extracts from fanconi anemia fibroblasts" 276 : 9543-9549, 2001

14 "DNA double-strand break repair by homologousrecombination" 383 : 873-892, 2002

15 "Convergence of the fanconi anemia and ataxia telangiectasia signaling pathways" 109 : 459-472, 2002

16 "Brca1 controls homology-directed DNA repair." 4 : 511-518, 1999

17 "Biallelic inactivation of BRCA2 in Fanconi anemia" 297 : 606- 609, 2002

18 "BRCA2 is required for homology-directed repair of chromosomal breaks" 7: : 263-272, 2001

19 "BRCA2 function in DNA bindingand recombination from a BRCA2-DSS1-ssDNAstructure" 297 : 1837--1848, 2002

20 "Asociation of bialelic BRCA2/FANCD1 mutations with spontaneous chromosomal instability and solid tumors of childhood" 103 : 2554-2559, 2004

21 "A novel ubiquitin ligase is deficient in Fanconi anemia" 35 : 165-170, 2003

22 "A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts" 32: : 3248-3257, 2004

23 "A DNA double strand break repair defect in Fanconi anemia fibroblasts" 277 : 46243-46247, 2002