녹각교 함유 인공피부를 이용한 피부독성도의 검사 = Assessment of Skin Toxicity Using Skin Equivalents Containing Cervi cornus Colla

다국어 초록 (Multilingual Abstract)

To substitute animal test, skin equivalents (SEs) have been developed for skin irritation and corrosion test. Recently, we have developed new SEs containing Cervi cornus Colla (CCC). In the present study, we used the SEs for cutaneous cytotoxicity test. Sodium dodecylsulfate (SDS) or sodium carbonate was applied to the SEs, and the epidermal damage by H&E and immunohistochemical stains was evaluated. Our results showed that SDS or sodium carbonate affected the epidermal part of SEs containing CCC in a dose-dependent manner and decreased the expression of p63. It is concluded that SEs containing CCC could be used for an alternative model of animal test and would be greatly helpful in the development of in vitro irritation and corrosion test.

참고문헌 (Reference)

1 A. B. Truong, "p63 regulates proliferation and differentiation of developmentally mature keratinocytes" 20 : 3185-, 2006

2 G. Pellegrini, "p63 identifies keratinocyte stem cells" 98 : 3156-, 2001

3 Y. Hijikata, "Treatment for intractable anemia with the traditional Chinese medicines Hominis Placenta and Cervi Cornus Colla (deer antler glue)" 2 : 83-, 2009

4 J. H. Draize, "Toxicity and primary irritation of some chemical compounds following oral administration and skin application" 5 : 174-, 1946

5 R. Roguet, "The use of in vitro reconstituted human skin in dermotoxicity testing" 8 : 635-, 1994

6 E. Bell, "Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro" 76 : 1274-, 1979

7 R. H. Rice, "Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions" 18 : 681-, 1979

8 C. Augustin, "Pharmacotoxicological applications of an equivalent dermis: three measurements of cytotoxicity" 11 : 167-, 1995

9 C. H. Yao, "Osteogenic potential using a malleable, biodegradable composite added traditional Chinese medicine: in vitro and in vivo evaluations" 34 : 873-, 2006

10 윤상웅, "Living skin equivalents를 이용한 피부독성도의 검사" 대한피부과학회 41 (41): 308-313, 2003

11 R. M. Greco, "Hyaluronic acid stimulates human fibroblast proliferation within a collagen matrix" 177 : 465-, 1998

12 C. H. Huh, "Effects of mesenchymal stem cells in the reconstruction of skin equivalents" 46 : 217-, 2007

13 H. J. Cho, "Effects of hair follicle dermal sheath cells in the reconstruction of skin equivalents" 35 : 74-, 2004

14 J. Kim, "Effects of Cervi cornus Colla (deer antler glue) in the reconstruction of a skin equivalent model" 305 : 85-, 2013

15 U. F. Schaefer, "Drug Absorption Studies: In Situ, in Vitro, and in Silico Models" Springer US press 3-, 2008

16 M. A. Perkins, "Development of an in vitro method for skin corrosion testing" 31 : 9-, 1996

17 Y. E. Kim, "8. Y. E. Kim, S. K. Lee, and U. C. Yoon, Studies on the Components and biological functions of animal hard tissue" 6 : 13-, 1973