원산지별 감초 추출물의 항산화 활성 비교 연구 = Comparative Study on Antioxidative Activity of Glycyrrhiza uralensis and Glycyrrhiza glabra Extracts by Country of Origin

다국어 초록 (Multilingual Abstract)

In this work, comparative study on antioxidative activities of extracts from Glycyrrhiza uralensis (G. uralensis) produced in Korea and in China and Glycyrrhiza glabra (G. glabra) produced in Uzbekistan was conducted. Among three origins, 50% ethanol extracts (21.15 μg/mL), ethyl acetate fraction (29.15 μg/mL) and aglycone fraction (3.26 μg/mL) of G. uralensis from Korea showed the higher free radical (1,1-phenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC₅₀) than extracts from other origins. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) of extracts from three origins on ROS generated in Fe³⁺-EDTA/H₂O₂ system were investigated using luminol-dependent chemiluminescence assay 50% ethanol extract (1.00 μg/mL) and ethyl acetate fraction (0.34 μg/mL) of G. uralensis from China showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of G. uralensis and G. glabra extracts on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. 50% ethanol extract and aglycone fraction of G. uralensis and G. glabra extracts from three origins showed cellular protective effects in a concentration dependent manner (5 ∼ 50 μg/mL). Aglycone fraction of G. uralensis from Korea (τ₅₀ = 847.4 min)especially showed cellular protective effects four times higher than that from China (τ₅₀ = 194.3 min). These results indicate that G. uralensis and G. glabra extracts, which have been used as whitening agent, could be applicable to functional cosmetic ingredient as a natural antioxidant. Judging from the prominent cellular protecitve effects, it is concluded that G. uralensis and G. glabra extracts can protect the skin from ¹O₂ and various ROS induced by UV.