감태 효소 추출물 및 폴리페놀 추출물의 생리활성에 관한 연구 = Biological Potential of Enzymatic and Polyphenol Extracts from Ecklonia cava

다국어 초록 (Multilingual Abstract)

To investigate the efficacy of enzymatic extract of Ecklonia cava and its polyphenol extract (AG-DK) as cosmetic ingredients, the anti-oxidative effect, anti-glycation effect, anti-melanogenic effect, and anti-inflammatory effect of the extracts were evaluated in vitro. The enzymatic extract of E. cava (SC50 42.9 ppm) and AG-DK (SC50 6.4 ppm) showed a strong DPPH free radical scavenging activity. The anti-glycation ability of the enzymatic extract of E. cava and AG-DK was tested using bovine serum albumin (BSA), which inhibited the formation of advanced glycation end-products (AGEs) in the BSA/glucose system. The enzymatic extract of E. cava (IC50 97.2 ppm) and AG-DK (IC50 7 ppm) had inhibitory effects on tyrosinase activity. Moreover, the enzymatic extract of E. cava and AG-DK had an anti-inflammatory effect through the inhibition of nitricoxide (NO) and prostaglandin E2 (PGE₂). These findings suggest that the enzymatic extract of E. cava and AG-DK can be applied to skin-care products as cosmetic ingredients.

참고문헌 (Reference)

1 Y. J. Kim, "Tyrosinase inhibitors from natural and synthetic sources: Structure, inhibition mechanism and perspective for the future" 62 : 1707-, 2005

2 K. A. Kang, "Triphlorethol-A induces heme oxygenase-1 via activation of ERK and NF-E2 related factor 2 transcription factor" 581 : 2000-, 2007

3 K. A. Kang, "Triphlorethol-A from Ecklonia cava protects V79-4 lung fibroblast against hydrogen peroxide induced cell damage" 39 : 883-, 2005

4 V. K. Dhargalkar, "Southern Ocean seaweeds: A resource for exploration in food and drugs" 287 : 229-, 2009

5 K. S. Prabhu, "Selenium deficiency increases the expression of inducible nitric oxide synthase in RAW 264.7 macrophages: Role of nuclear factor-κB in up-regulation" 366 : 203-, 2002

6 N. Matsuura, "Screening system for the Maillard reaction inhibitor from natural product extracts" 48 : 520-, 2002

7 K. Iozumi, "Role of tyrosinase as the determinant of pigmentation in cultured human melanocytes free" 100 : 806-, 1993

8 D. Latorre, "Reciprocal Interactions between Lactoferrin and Bacterial Endotoxins and Their Role in the Regulation of the Immune Response" 2 : 54-, 2010

9 T. Mossman, "Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays" 65 : 55-, 1983

10 C. C. Wang, "Protective effect of dried safflower petal aqueous extract and its main constituent, carthamus yellow, against lipopolysaccharideinduced inflammation in RAW 264.7 macrophages" 91 : 218-, 2011

11 M. M. Kim, "Phlorotannins in Ecklonia cava extract inhibit matrix metalloproteinase activity" 79 : 1436-, 2006

12 P. Aroca, "Melanin biosynthesis patterns following hormonal stimulation" 268 : 25650-, 1993

13 M. J. Ahn, "Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava" 27 : 544-, 2004

14 C. S. Kong, "Induction of apoptosis by phloroglucinol derivative from Ecklonia cava in MCF-7 human breast cancer cells" 47 : 1653-, 2009

15 T. Kuda, "Fate of watersoluble polysaccharides in dried Chorda filum a brown alga during water washing" 15 : 3-, 2002

16 You-Jin Jeon, "Cytotoxic Activities of Green and Brown Seaweeds Collected from Jeju Island against Four Tumor Cell Lines" 한국식품영양과학회 11 (11): 17-24, 2006

17 Soo-Jin Heo, "Antioxidative Effect of Proteolytic Hydrolysates from Ecklonia cava onRadical Scavenging Using ESR and H2O2-induced DNA Damage" 한국식품과학회 14 (14): 614-620, 2005

18 S. J. Heo, "Antioxidant activity of enzymatic extracts from brown seaweeds" 18 : 71-, 2003

19 S. J. Heo, "Antioxidant activities of enzymatic extracts from brown seaweeds" 96 : w1613-, 2005

20 Y. Athukorala, "Anticoagulative polysaccharide froman enzymatic hydrolysate of Ecklonia cava, Carbohydr" 66 : 184-, 2006

21 K. Matsubara, "An anticoagulant proteoglycan from the marine green alga, Codium pugniformis" 12 : 9-, 2000