사람唾液 Superoxide Dismutase의 精製 및 性狀 = Purification and Some Properties of Superoxide Dismutase from Human Saliva

다국어 초록 (Multilingual Abstract)

The purification and some properties of superoxide dismutase(O_(2-)-O_(2-) oxidoreductase, ECI. 15.1.1) of human saliva have been studied. Human saliva was collected from medical students, male and female together with smoking male students. The activity of superoxide dismutase was measured by the method of McCord and Fridovich. The superoxide dismutase was purified by ammonium sulfate precifitation, DEAE-cellulose and sephadex C-100 column chromatography. The results are summerized as follows :
1. Human saliva contain measurable amounts of superoxide dismutase.
2. The activities of superoxide dismutase. were 0.72U/㎖ in male and 0.73U/㎖ in female saliva.
The activity of this enzyme was 0.81U/㎖ in smoker. Accodingly, there was no recognizabIe differences in the enzyme activity between smoker and non-smoker.
4. The human salivary superoxide dismutase was purified approximately 90 folds, and the molecular weight of the purified enzyme was estimated to be approximately 32,000 by gel filtretion.
4. Purified salivary superoxide dismutase has contained 1.83 atoms of copper and 2.00 atoms of zinc per molecule and was strikingly similar to the superoxide dismutase previonsly isolated from other cukaryotes by other investigatury.
5. The ultraviolet absorption spectrum of purified salivan- superoxide dismutase was 268㎚ which was similar to the value previously seen with the cytosolic enzyme of eukaryotes.